Literature DB >> 8582141

Use of nondenaturing silver-stained polyacrylamide gel analysis of polymerase chain reaction amplification products for the differential diagnosis of Leptospira interrogans infection.

M A Oliveira1, O L Caballero, E Dias Neto, M C Koury, A J Romanha, J Carvalho, R A Hartskeerl, A J Simpson.   

Abstract

A 285-bp DNA fragment was amplified using the polymerase chain reaction from 38 Leptospira serovars of six different genomic species. The fragments amplified exhibited differential mobilities on nondenaturing polyacrylamide gels resulting from sequence-dependent conformational alterations. Leptospira interrogans serovars could be distinguished from those of other species on this basis.

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Year:  1995        PMID： 8582141     DOI： 10.1016/0732-8893(95)00145-3

Source DB:  PubMed          Journal:  Diagn Microbiol Infect Dis        ISSN： 0732-8893            Impact factor:   2.803


  3 in total

Review 1.  Leptospirosis.

Authors:  P N Levett
Journal:  Clin Microbiol Rev       Date:  2001-04       Impact factor: 26.132

2.  Identification of pathogenic Leptospira genospecies by continuous monitoring of fluorogenic hybridization probes during rapid-cycle PCR.

Authors:  T H Woo; B K Patel; L D Smythe; M L Symonds; M A Norris; M F Dohnt
Journal:  J Clin Microbiol       Date:  1997-12       Impact factor: 5.948

3.  Use of a New High Resolution Melting Method for Genotyping Pathogenic Leptospira spp.

Authors:  Florence Naze; Amélie Desvars; Mathieu Picardeau; Pascale Bourhy; Alain Michault
Journal:  PLoS One       Date:  2015-07-08       Impact factor: 3.240
  3 in total

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