| Literature DB >> 8581299 |
G Rettenberger1, W Zimmermann, C Klett, U Zechner, H Hameister.
Abstract
PCR with primers specific for the murine B1 consensus sequence allows amplification of DNA from murine sources. We have used B1-PCR for amplifying yeast artificial chromosome (YAC) DNA which can be used to localize single YACs by fluorescence in situ hybridization. The genes for the pregnancy-specific glycoproteins Cea2 and Cea4, both belonging to the large carcinoembryonic antigen gene family, were localized by chromosomal in situ suppression hybridization of three YAC clones to murine chromosome 7A2-A3. This was facilitated by the use of the mouse lymphoma cell line WMP/WMP which contains nine pairs of Robertsonian fusion chromosomes.Entities:
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Year: 1995 PMID: 8581299 DOI: 10.1007/bf00713961
Source DB: PubMed Journal: Chromosome Res ISSN: 0967-3849 Impact factor: 5.239