| Literature DB >> 8581129 |
S Sumi1, K Kidouchi, S Ohba, Y Wada.
Abstract
An automated screening system for purine and pyrimidine metabolism disorders using high-performance liquid chromatography (HPLC) with column switching is described. The system consists of a reversed-phase column, a cation-exchange column, a column switch, four sets of ultraviolet absorbance detectors, a microcomputer and other conventional equipment. As this system permits the simultaneous determination of urinary orotic acid, uracil, dihydrouracil, pseudouridine, xanthine, 2,8-dihydroxyadenine and succinyladenosine, it offers a useful method for the detection of orotic aciduria, dihydropyrimidine dehydrogenase deficiency, dihydropyrimidinuria, xanthinuria, adenine phosphoribosyltransferase deficiency and adenylosuccinase deficiency.Entities:
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Year: 1995 PMID： 8581129 DOI： 10.1016/0378-4347(95)00228-b
Source DB: PubMed Journal: J Chromatogr B Biomed Appl ISSN： 1572-6495