Literature DB >> 8580854

Crystallization and preliminary X-ray analysis of L-2-haloacid dehalogenase from Xanthobacter autotrophicus GJ10.

I S Ridder1, H J Rozeboom, J Kingma, D B Janssen, B W Dijkstra.   

Abstract

Haloacid dehalogenases are enzymes that cleave carbon-chlorine or carbon-bromine bonds of 2-haloalkanoates. X-ray-quality crystals of L-2-haloacid dehalogenase from the 1,2-dichloroethane-degrading bacterium Xanthobacter autotrophicus GJ10 have been grown at room temperature from 20% PEG 8000, 200 mM sodium formate at pH 6.8-7.0, using macroseeding techniques. The crystals, which diffract in the X-ray beam up to 2.0 A resolution, belong to the spacegroup C2221. Cell parameters are a = 58.8 A, b = 93.1 A, c = 84.2 A. A native data set to 2.3 A has been collected, with a completeness of 97% and an Rsym of 6.0%.

Entities:  

Mesh：

Substances：

Year:  1995        PMID： 8580854      PMCID： PMC2143032          DOI： 10.1002/pro.5560041220

Source DB:  PubMed          Journal:  Protein Sci        ISSN： 0961-8368            Impact factor:   6.725


  9 in total

1.  Carbon-halogen bond cleavage. 3. Studies on bacterial halidohrolases.

Authors:  P Goldman; G W Milne; D B Keister
Journal:  J Biol Chem       Date:  1968-01-25       Impact factor: 5.157

2.  Solvent content of protein crystals.

Authors:  B W Matthews
Journal:  J Mol Biol       Date:  1968-04-28       Impact factor: 5.469

3.  Comprehensive site-directed mutagenesis of L-2-halo acid dehalogenase to probe catalytic amino acid residues.

Authors:  T Kurihara; J Q Liu; V Nardi-Dei; H Koshikawa; N Esaki; K Soda
Journal:  J Biochem       Date:  1995-06       Impact factor: 3.387

4.  Chlorinated hydrocarbons and the environment.

Authors:  G McConnell; D M Ferguson; C R Pearson
Journal:  Endeavour       Date:  1975-01       Impact factor: 0.444

5.  Cloning and sequence analysis of a plasmid-encoded 2-haloacid dehalogenase gene from Pseudomonas putida No. 109.

Authors:  H Kawasaki; T Toyama; T Maeda; H Nishino; K Tonomura
Journal:  Biosci Biotechnol Biochem       Date:  1994-01       Impact factor: 2.043

6.  Degradation of halogenated aliphatic compounds by Xanthobacter autotrophicus GJ10.

Authors:  D B Janssen; A Scheper; L Dijkhuizen; B Witholt
Journal:  Appl Environ Microbiol       Date:  1985-03       Impact factor: 4.792

7.  Purification and characterization of thermostable and nonthermostable 2-haloacid dehalogenases with different stereospecificities from Pseudomonas sp. strain YL.

Authors:  J Q Liu; T Kurihara; A K Hasan; V Nardi-Dei; H Koshikawa; N Esaki; K Soda
Journal:  Appl Environ Microbiol       Date:  1994-07       Impact factor: 4.792

8.  Crystallographic analysis of the catalytic mechanism of haloalkane dehalogenase.

Authors:  K H Verschueren; F Seljée; H J Rozeboom; K H Kalk; B W Dijkstra
Journal:  Nature       Date:  1993-06-24       Impact factor: 49.962

9.  Characterization of the haloacid dehalogenase from Xanthobacter autotrophicus GJ10 and sequencing of the dhlB gene.

Authors:  J van der Ploeg; G van Hall; D B Janssen
Journal:  J Bacteriol       Date:  1991-12       Impact factor: 3.490
  9 in total
  1 in total

Review 1.  l-2-Haloacid dehalogenase (DehL) from Rhizobium sp. RC1.

Authors:  Aliyu Adamu; Roswanira Abdul Wahab; Fahrul Huyop
Journal:  Springerplus       Date:  2016-05-20
  1 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.