E C Pirtle1, G W Beran. 1. Department of Microbiology, Immunology, and Preventive Medicine, College of Veterinary Medicine, Iowa State University, Ames 50011, USA.
Abstract
OBJECTIVE: To determine the survival of porcine reproductive and respiratory syndrome virus (PRRSV) on nonliving substances (fomites) at 25 to 27 C. DESIGN: Prospective controlled study. SAMPLE POPULATION: 3 solid, 6 porous, and 7 liquid fomites. PROCEDURE: The fomites were contaminated with known concentrations of PRRSV. Samples for virus isolation were obtained on day 0 through day 11, assayed in cell cultures, and stained with fluorescent antibody conjugate. RESULTS: The virus was recovered only on day-0 samples of alfalfa, wood shavings, straw, plastic, boot rubber, and stainless steel. Virus was isolated from city water through day 11, from well water through day 9, and from 2 buffer solutions for 4 and 6 days. The virus was isolated only on day 0 from swine saliva, urine, and fecal slurry. CLINICAL IMPLICATIONS: Results indicated that PRRSV is a fairly labile virus, but because of its duration of viability in water, contamination of drinking water and lagoons by PRRSV-shedding swine would serve as sources of virus to infect susceptible swine.
OBJECTIVE: To determine the survival of porcine reproductive and respiratory syndrome virus (PRRSV) on nonliving substances (fomites) at 25 to 27 C. DESIGN: Prospective controlled study. SAMPLE POPULATION: 3 solid, 6 porous, and 7 liquid fomites. PROCEDURE: The fomites were contaminated with known concentrations of PRRSV. Samples for virus isolation were obtained on day 0 through day 11, assayed in cell cultures, and stained with fluorescent antibody conjugate. RESULTS: The virus was recovered only on day-0 samples of alfalfa, wood shavings, straw, plastic, boot rubber, and stainless steel. Virus was isolated from city water through day 11, from well water through day 9, and from 2 buffer solutions for 4 and 6 days. The virus was isolated only on day 0 from swine saliva, urine, and fecal slurry. CLINICAL IMPLICATIONS: Results indicated that PRRSV is a fairly labile virus, but because of its duration of viability in water, contamination of drinking water and lagoons by PRRSV-shedding swine would serve as sources of virus to infect susceptible swine.
Authors: Scott Dee; John Deen; Kurt Rossow; Carrie Wiese; Satoshi Otake; Han Soo Joo; Carlos Pijoan Journal: Can J Vet Res Date: 2002-10 Impact factor: 1.310
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Authors: Scott A Dee; Fernando V Bauermann; Megan C Niederwerder; Aaron Singrey; Travis Clement; Marcelo de Lima; Craig Long; Gilbert Patterson; Maureen A Sheahan; Ana M M Stoian; Vlad Petrovan; Cassandra K Jones; Jon De Jong; Ju Ji; Gordon D Spronk; Luke Minion; Jane Christopher-Hennings; Jeff J Zimmerman; Raymond R R Rowland; Eric Nelson; Paul Sundberg; Diego G Diel Journal: PLoS One Date: 2018-03-20 Impact factor: 3.240
Authors: Rebecca K Gordon; Ingrid K Kotowski; Kari F Coulson; Donald Link; Alexandra MacKenzie; Joyce Bowling-Heyward Journal: Front Vet Sci Date: 2019-08-22