Literature DB >> 8573475

Crystallization and preliminary X-ray analysis of Borrelia burgdorferi outer surface protein A (OspA) complexed with a murine monoclonal antibody Fab fragment.

H Li1, C L Lawson.   

Abstract

The Borrelia burgdorferi outer surface lipoprotein OspA is a current focus for vaccine development to prevent Lyme disease infection. A soluble, recombinant form of the protein lacking the amino-terminal lipid membrane anchor was cocrystallized with the Fab fragment of an agglutinating mouse monoclonal antibody. The crystals belong to space group P2(1)2(1)2(1), with a = 90.0 A, b = 91.9 A, and c = 102.9 A and they were found to diffract to a maximum resolution of 2.8 A using synchrotron radiation.

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Year:  1995        PMID： 8573475     DOI： 10.1006/jsbi.1995.1058

Source DB:  PubMed          Journal:  J Struct Biol        ISSN： 1047-8477            Impact factor:   2.867


  6 in total

1.  Atomic-resolution crystal structure of Borrelia burgdorferi outer surface protein A via surface engineering.

Authors:  Koki Makabe; Valentina Tereshko; Grzegorz Gawlak; Shude Yan; Shohei Koide
Journal:  Protein Sci       Date:  2006-07-05       Impact factor: 6.725

2.  Crystal structure of Lyme disease antigen outer surface protein A complexed with an Fab.

Authors:  H Li; J J Dunn; B J Luft; C L Lawson
Journal:  Proc Natl Acad Sci U S A       Date:  1997-04-15       Impact factor: 11.205

3.  Crystal structure of neurotropism-associated variable surface protein 1 (Vsp1) of Borrelia turicatae.

Authors:  Catherine L Lawson; Brian H Yung; Alan G Barbour; Wolfram R Zückert
Journal:  J Bacteriol       Date:  2006-06       Impact factor: 3.490

4.  Agglutination of Borreliella burgdorferi by Transmission-Blocking OspA Monoclonal Antibodies and Monovalent Fab Fragments.

Authors:  Amber M Frye; Monir Ejemel; Lisa Cavacini; Yang Wang; Michael J Rudolph; Renjie Song; Nicholas J Mantis
Journal:  Infect Immun       Date:  2022-08-24       Impact factor: 3.609

Review 5.  Outer membrane proteins of pathogenic spirochetes.

Authors:  Paul A Cullen; David A Haake; Ben Adler
Journal:  FEMS Microbiol Rev       Date:  2004-06       Impact factor: 16.408

6.  Tick receptor for outer surface protein A from Ixodes ricinus - the first intrinsically disordered protein involved in vector-microbe recognition.

Authors:  Anna Urbanowicz; Dominik Lewandowski; Kamil Szpotkowski; Marek Figlerowicz
Journal:  Sci Rep       Date:  2016-04-26       Impact factor: 4.379
  6 in total

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