L Mazur1. 1. Department of Animal Physiology, Jagiellonian University, Cracow, Poland.
Abstract
PURPOSE: The protective effect of the sulphur-containing agents, MEA (cysteamine HCl), AET (2-aminoethyl-isothiouronium Br.HBr), and WR-2721 (S-2/3-aminopropylamino/ethylphosphorothioic acid) against induction of micronuclei in the erythropoietic system by X-rays was assessed. METHODS: The mouse peripheral blood micronucleus assay was applied. The frequency of micronucleated polychromatic erythrocytes (MNPCE) in the peripheral blood of adult male Swiss mice was determined 24 h after MEA, AET, or WR-2721 treatment, at a dose of 200 mg/kg body weight, and whole-body X-ray exposure to a dose of 6 Gy. RESULTS: After X-irradiation of mice, the number of MNPCE was distinctly increased. MEA, AET, or WR-2721 administration, 15 min prior to X-irradiation resulted in a reduction of the X-ray-induced elevation of the frequency of micronuclei. So, the radioprotection of the sulphur-containing drugs against the induction of MNPCE in the erythropoietic system by X-rays was shown. CONCLUSION: The mouse peripheral blood micronucleus test can be used to screen potential radioprotective agents.
PURPOSE: The protective effect of the sulphur-containing agents, MEA (cysteamine HCl), AET (2-aminoethyl-isothiouronium Br.HBr), and WR-2721 (S-2/3-aminopropylamino/ethylphosphorothioic acid) against induction of micronuclei in the erythropoietic system by X-rays was assessed. METHODS: The mouse peripheral blood micronucleus assay was applied. The frequency of micronucleated polychromatic erythrocytes (MNPCE) in the peripheral blood of adult male Swiss mice was determined 24 h after MEA, AET, or WR-2721 treatment, at a dose of 200 mg/kg body weight, and whole-body X-ray exposure to a dose of 6 Gy. RESULTS: After X-irradiation of mice, the number of MNPCE was distinctly increased. MEA, AET, or WR-2721 administration, 15 min prior to X-irradiation resulted in a reduction of the X-ray-induced elevation of the frequency of micronuclei. So, the radioprotection of the sulphur-containing drugs against the induction of MNPCE in the erythropoietic system by X-rays was shown. CONCLUSION: The mouse peripheral blood micronucleus test can be used to screen potential radioprotective agents.
Authors: Miguel Alcaraz; Amparo Olivares; Daniel Gyingiri Achel; José Antonio García-Gamuz; Julián Castillo; Miguel Alcaraz-Saura Journal: Antioxidants (Basel) Date: 2021-12-30