Literature DB >> 8570643

In vivo examination of membrane protein localization and degradation with green fluorescent protein.

R Y Hampton1, A Koning, R Wright, J Rine.   

Abstract

To test the utility of green fluorescent protein (GFP) as an in vivo reporter protein when fused to a membrane domain, we made a fusion protein between yeast hydroxymethylglutaryl-CoA reductase and GFP. Fusion proteins displayed spatial localization and regulated degradation consistent with the native hydroxymethylglutaryl-CoA reductase proteins. Thus, GFP should be useful in the study of both membrane protein localization and protein degradation in vivo.

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Year:  1996        PMID： 8570643      PMCID： PMC40142          DOI： 10.1073/pnas.93.2.828

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  11 in total

1.  The regulated degradation of 3-hydroxy-3-methylglutaryl-CoA reductase requires a short-lived protein and occurs in the endoplasmic reticulum.

Authors:  K T Chun; S Bar-Nun; R D Simoni
Journal:  J Biol Chem       Date:  1990-12-15       Impact factor: 5.157

Review 2.  Fluorescent labeling of endoplasmic reticulum.

Authors:  M Terasaki
Journal:  Methods Cell Biol       Date:  1989       Impact factor: 1.441

3.  DiOC6 staining reveals organelle structure and dynamics in living yeast cells.

Authors:  A J Koning; P Y Lum; J M Williams; R Wright
Journal:  Cell Motil Cytoskeleton       Date:  1993

4.  Transmission electron microscopy and immunocytochemical studies of yeast: analysis of HMG-CoA reductase overproduction by electron microscopy.

Authors:  R Wright; J Rine
Journal:  Methods Cell Biol       Date:  1989       Impact factor: 1.441

5.  Green fluorescent protein as a marker for gene expression.

Authors:  M Chalfie; Y Tu; G Euskirchen; W W Ward; D C Prasher
Journal:  Science       Date:  1994-02-11       Impact factor: 47.728

6.  KAR2, a karyogamy gene, is the yeast homolog of the mammalian BiP/GRP78 gene.

Authors:  M D Rose; L M Misra; J P Vogel
Journal:  Cell       Date:  1989-06-30       Impact factor: 41.582

7.  Structure of the yeast endoplasmic reticulum: localization of ER proteins using immunofluorescence and immunoelectron microscopy.

Authors:  D Preuss; J Mulholland; C A Kaiser; P Orlean; C Albright; M D Rose; P W Robbins; D Botstein
Journal:  Yeast       Date:  1991-12       Impact factor: 3.239

8.  Chemical structure of the hexapeptide chromophore of the Aequorea green-fluorescent protein.

Authors:  C W Cody; D C Prasher; W M Westler; F G Prendergast; W W Ward
Journal:  Biochemistry       Date:  1993-02-09       Impact factor: 3.162

9.  Increased amounts of HMG-CoA reductase induce "karmellae": a proliferation of stacked membrane pairs surrounding the yeast nucleus.

Authors:  R Wright; M Basson; L D'Ari; J Rine
Journal:  J Cell Biol       Date:  1988-07       Impact factor: 10.539

10.  Regulated degradation of HMG-CoA reductase, an integral membrane protein of the endoplasmic reticulum, in yeast.

Authors:  R Y Hampton; J Rine
Journal:  J Cell Biol       Date:  1994-04       Impact factor: 10.539
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  41 in total

1.  The role of the 3-hydroxy 3-methylglutaryl coenzyme A reductase cytosolic domain in karmellae biogenesis.

Authors:  D A Profant; C J Roberts; A J Koning; R L Wright
Journal:  Mol Biol Cell       Date:  1999-10       Impact factor: 4.138

2.  Green fluorescent protein functions as a reporter for protein localization in Escherichia coli.

Authors:  B J Feilmeier; G Iseminger; D Schroeder; H Webber; G J Phillips
Journal:  J Bacteriol       Date:  2000-07       Impact factor: 3.490

3.  Functional expression, quantification and cellular localization of the Hxt2 hexose transporter of Saccharomyces cerevisiae tagged with the green fluorescent protein.

Authors:  A L Kruckeberg; L Ye; J A Berden; K van Dam
Journal:  Biochem J       Date:  1999-04-15       Impact factor: 3.857

4.  In vivo action of the HRD ubiquitin ligase complex: mechanisms of endoplasmic reticulum quality control and sterol regulation.

Authors:  R G Gardner; A G Shearer; R Y Hampton
Journal:  Mol Cell Biol       Date:  2001-07       Impact factor: 4.272

5.  Generation of T-DNA tagging lines with a bidirectional gene trap vector and the establishment of an insertion-site database.

Authors:  Choong-Hwan Ryu; Jung-Hwa You; Hong-Gyu Kang; Junghe Hur; Young-Hea Kim; Min-Jung Han; Kyungsook An; Byoung-Chull Chung; Choon-Hwan Lee; Gynheung An
Journal:  Plant Mol Biol       Date:  2004-03       Impact factor: 4.076

6.  Using bleach-chase to measure protein half-lives in living cells.

Authors:  Naama Geva-Zatorsky; Irina Issaeva; Avi Mayo; Ariel Cohen; Erez Dekel; Tamar Danon; Lydia Cohen; Yuvalal Liron; Uri Alon; Eran Eden
Journal:  Nat Protoc       Date:  2012-03-29       Impact factor: 13.491

7.  Subcellular localization of Arabidopsis 3-hydroxy-3-methylglutaryl-coenzyme A reductase.

Authors:  Pablo Leivar; Víctor M González; Susanna Castel; Richard N Trelease; Carmen López-Iglesias; Montserrat Arró; Albert Boronat; Narciso Campos; Albert Ferrer; Xavier Fernàndez-Busquets
Journal:  Plant Physiol       Date:  2004-12-23       Impact factor: 8.340

8.  Vascular endothelial tissue factor pathway inhibitor kinetics in culture following exposure to DX-9065a--a selective and direct factor Xa inhibitor.

Authors:  Richard C Becker; John H Alexander; Youfu Li; Thomas Robertson; Satoshi Kunitada; Frederick A Spencer; Hongqiu Yang; Robert A Harrington
Journal:  J Thromb Thrombolysis       Date:  2004-12       Impact factor: 2.300

9.  Genetic and structural analysis of Hmg2p-induced endoplasmic reticulum remodeling in Saccharomyces cerevisiae.

Authors:  Christine M Federovitch; Ying Z Jones; Amy H Tong; Charles Boone; William A Prinz; Randolph Y Hampton
Journal:  Mol Biol Cell       Date:  2008-07-30       Impact factor: 4.138

10.  Lipid-membrane affinity of chimeric metal-binding green fluorescent protein.

Authors:  V Prachayasittikul; C Isarankura Na Ayudhya; S Boonpangrak; H-J Galla
Journal:  J Membr Biol       Date:  2004-07-01       Impact factor: 1.843
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