| Literature DB >> 8570085 |
N Dutrait1, M Culcasi, C Cazevieille, S Pietri, P Tordo, C Bonne, A Muller.
Abstract
Cultured rat retinal neurons exposed to kainate produced free radicals, as demonstrated by electron spin resonance (ESR) spin trapping using the nitrone 5,5-dimethyl-1-pyrroline-N-oxide (DMPO) and the generation of DMPO hydroxyl adduct (DMPO-OH). This DMPO-OH production was abolished by EGTA, nitro-arginine and oxypurinol, suggesting that it was dependent on Ca2+ influx and subsequent activation of nitric oxide synthase and xanthine oxidase. Moreover, kainate induced a receptor-mediated Ca2+ influx and neuronal injury assessed by lactate dehydrogenase release. Neuroprotection afforded by nitro-arginine and oxypurinol shows that calcium-dependent free radical production plays a major role in kainate retinal toxicity.Entities:
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Year: 1995 PMID： 8570085 DOI： 10.1016/0304-3940(95)11948-v
Source DB: PubMed Journal: Neurosci Lett ISSN： 0304-3940 Impact factor: 3.046