Literature DB >> 8569766

Detection and genotyping of herpes simplex virus types 1 and 2 by polymerase chain reaction.

G Lucotte1, C Bathelier, V Lespiaux, C Bali, T Champenois.   

Abstract

A simple and rapid polymerase chain reaction (PCR) procedure was developed for simultaneous detection and typing of herpes simplex virus (HSV) types 1 and 2. It was possible to detect and type HSV using two primers pairs in a simultaneous double PCR reaction, where the type of HSV present was determined on the basis of an ethidium-bromide-stained band after agarose gel electrophoresis. This PCR assay was tested on about 500 clinical specimens.

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Year:  1995        PMID： 8569766     DOI： 10.1016/s0890-8508(95)91508-7

Source DB:  PubMed          Journal:  Mol Cell Probes        ISSN： 0890-8508            Impact factor:   2.365


  6 in total

1.  Clear detection and typing of herpes simplex virus types 1 and 2 by an indirect ELISA assay: comparison with three different combined methods--capture ELISA, restriction enzymes, and polymerase chain reaction.

Authors:  P Markoulatos; P Fountoucidou; G Marinakis; V Krikelis; N Spyrou; N Vamvakopoulos; M L Moncany
Journal:  J Clin Lab Anal       Date:  1997       Impact factor: 2.352

2.  Herpes simplex virus type 1-specific cytotoxic T-lymphocyte arming occurs within lymph nodes draining the site of cutaneous infection.

Authors:  C M Jones; S C Cose; R M Coles; A C Winterhalter; A G Brooks; W R Heath; F R Carbone
Journal:  J Virol       Date:  2000-03       Impact factor: 5.103

3.  Detection and typing of HSV-1, HSV-2, and VZV by a multiplex polymerase chain reaction.

Authors:  P Markoulatos; A Georgopoulou; C Kotsovassilis; P Karabogia-Karaphillides; N Spyrou
Journal:  J Clin Lab Anal       Date:  2000       Impact factor: 2.352

4.  Evaluation of herpes simplex detection in corneal scrapings by three molecular methods.

Authors:  Amina Mostafa Abd El-Aal; Maysaa El Sayed; Eglal Mohammed; Mohammed Ahmed; Mona Fathy
Journal:  Curr Microbiol       Date:  2006-04-01       Impact factor: 2.188

5.  Rapid cytotoxic T lymphocyte activation occurs in the draining lymph nodes after cutaneous herpes simplex virus infection as a result of early antigen presentation and not the presence of virus.

Authors:  Scott N Mueller; Claerwen M Jones; Chris M Smith; William R Heath; Francis R Carbone
Journal:  J Exp Med       Date:  2002-03-04       Impact factor: 14.307

6.  Genital infection with herpes simplex virus types 1 and 2 in women from natal, Brazil.

Authors:  Cleine Aglacy Nunes Miranda; Erika Galvão Lima; Diego Breno Soares de Lima; Ricardo Ney Oliveira Cobucci; Maria da Conceição de Mesquita Cornetta; Thales Allyrio Araújo de Medeiros Fernandes; Paulo Roberto Medeiros de Azevedo; Jenner Chrystian Veríssimo de Azevedo; Josélio Maria Galvão de Araújo; José Veríssimo Fernandes
Journal:  ISRN Obstet Gynecol       Date:  2014-03-11
  6 in total

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