| Literature DB >> 8567985 |
M Mogi1, H Inagaki, K Kojima, M Minami, M Harada.
Abstract
A sensitive sandwich enzyme immunoassay (EIA) for transforming growth factor-alpha (TGF-alpha) utilizing a polyclonal antibody that recognizes limited epitopes of both human TGF-alpha and rat TGF-alpha in combination with a monoclonal anti-TGF-alpha IgG1 galactosidase conjugate was developed. This assay shows no cross-reactivity with human epidermal growth factor. We can quantify the TGF-alpha level in not only human TGF-alpha (detection limit: 1 pg/ml), but also rat TGF-alpha (detection limit: 10 pg/ml) by virtue of cross-reactivity. Employing this assay system, we demonstrated that TGF-alpha is present in both human submandibular glands and submandibular/sublingual saliva.Entities:
Mesh：
Substances：
Year: 1995 PMID： 8567985 DOI： 10.1080/15321819508013569
Source DB: PubMed Journal: J Immunoassay ISSN： 0197-1522