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Literature DB >> 8567888

Effect of three concentration techniques on viability of Cryptosporidium parvum oocysts recovered from bovine feces.

Abstract

Bovine fecal samples (1 g) negative for Cryptosporidium sp. oocysts were seeded with 7 x 10(4) Cryptosporidium parvum oocysts and purified by either water-ether concentration, sucrose density flotation, or zinc sulfate flotation to evaluate oocyst recovery. The effect of these purification techniques on the viability of recovered oocysts was also evaluated. Significantly higher numbers of seeded oocysts were recovered by water-ether concentration (recovery rate, 46 to 75%) than by sucrose density (24 to 65%) or zinc sulfate (22 to 41%) flotation methods. In addition, water-ether concentration did not exert a significant effect on the viability of the population of oocysts recovered, whereas sucrose density flotation and zinc sulfate flotation selectively concentrated viable oocysts. The water-ether concentration procedure is recommended for use in epidemiological studies in which both oocyst enumeration and viability assessment are required.

Entities: Chemical Species

Mesh：

Substances：
Fluorescent Dyes

Year: 1995 PMID： 8567888 PMCID： PMC228535 DOI： 10.1128/jcm.33.10.2592-2595.1995

Source DB: PubMed Journal: J Clin Microbiol ISSN： 0095-1137 Impact factor: 5.948

10 in total

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Authors: R Weber; R T Bryan; D D Juranek
Journal: J Clin Microbiol Date: 1992-11 Impact factor: 5.948

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Journal: J Clin Microbiol Date: 1986-01 Impact factor: 5.948

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Journal: J Clin Microbiol Date: 1991-07 Impact factor: 5.948

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Journal: J Infect Dis Date: 1983-12 Impact factor: 5.226

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Journal: Vet Parasitol Date: 1992-12 Impact factor: 2.738

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Journal: Parasitology Date: 1993-01 Impact factor: 3.234

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Journal: Appl Environ Microbiol Date: 1995-02 Impact factor: 4.792

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Journal: J Clin Microbiol Date: 1983-07 Impact factor: 5.948

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Journal: Appl Environ Microbiol Date: 1992-11 Impact factor: 4.792

10 in total

28 in total

1. Extraction-free, filter-based template preparation for rapid and sensitive PCR detection of pathogenic parasitic protozoa.

Authors: P A Orlandi; K A Lampel
Journal: J Clin Microbiol Date: 2000-06 Impact factor: 5.948

2. New method using sedimentation and immunomagnetic separation for isolation and enumeration of Cryptosporidium parvum oocysts and Giardia lamblia cysts.

Authors: Jaime Massanet-Nicolau
Journal: Appl Environ Microbiol Date: 2003-11 Impact factor: 4.792

3. Purification of Sarcocystis neurona sporocysts from opossum (Didelphis virginiana) using potassium bromide discontinuous density gradient centrifugation.

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Journal: Parasitol Res Date: 2003-02-11 Impact factor: 2.289

4. Effect of bovine manure on Cryptosporidium parvum oocyst attachment to soil.

Authors: Ewa Kuczynska; Daniel R Shelton; Yakov Pachepsky
Journal: Appl Environ Microbiol Date: 2005-10 Impact factor: 4.792

5. Incidence of cryptosporidiosis species in paediatric patients in Malawi.

Authors: T D Morse; R A B Nichols; A M Grimason; B M Campbell; K C Tembo; H V Smith
Journal: Epidemiol Infect Date: 2007-01-15 Impact factor: 2.451

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Authors: Valerie De Waele; Marco Berzano; Dirk Berkvens; Niko Speybroeck; Colm Lowery; Grace M Mulcahy; Thomas M Murphy
Journal: J Clin Microbiol Date: 2010-11-03 Impact factor: 5.948