Differential expression of ob mRNA in rat adipose tissues in response to insulin.

The recently cloned obese (ob) gene encodes a fat-specific mRNA and a protein which is believed to regulate satiety and metabolic rate so as to control fat storage. In the present study we investigated rat ob mRNA levels in different fat depots and the effects of insulin infusion on ob mRNA expression. Northern blot analysis showed the abdominal fat (epididymal and perirenal fat pads) had higher ob mRNA levels than subcutaneous fat. After a 2.5-hr infusion of insulin into fasted rats, ob mRNA levels were increased by 3.0 and 1.9-fold in epididymal and perirenal fat pads, respectively. However, no change of ob mRNA level was observed in the subcutaneous fat depot. These data demonstrate adipose depot site-specific expression of ob mRNA and support the hypothesis that insulin plays an important role in the expression of ob mRNA.