Literature DB >> 85610

Immunoelectrophoretic identification and purification of herpes simplex virus antigens released from infected cells in tissue culture.

B Norrild, B F Vestergaard.   

Abstract

Proteins released from herpes simplex virus type 1 (HSV-1)-and type 2 (HSV-2)-infected HEp-2 cells have been characterized by the crossed immunoelectrophoretic technique. Both HSV type-common and type-specific antigens were found in the tissue culture medium 24 h after infection. Antigen Ag-6, an HSV-1-specific antigen, was found in high concentration in the medium as compared to other HSV antigens released from HSV-1-infected cells. The HSV-2-specific antigens, Ag-4 and Ag-1, were released in molecular modifications with altered electrophoretic mobility as compared to their cellular counterparts. Purification of HSV antigens was performed by ion-exchange chromatography, and an HSV type-common antigen, Ag-11, and an HSV-2-specific antigen, Ag-4A, were isolated.

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Year:  1979        PMID： 85610     DOI： 10.1159/000149020

Source DB:  PubMed          Journal:  Intervirology        ISSN： 0300-5526            Impact factor:   1.763


  9 in total

1.  Herpes simplex virus glycoproteins: participation of individual herpes simplex virus type 1 glycoprotein antigens in immunocytolysis and their correlation with previously identified glycopolypeptides.

Authors:  B Norrild; S L Shore; A J Nahmias
Journal:  J Virol       Date:  1979-12       Impact factor: 5.103

2.  gA and gB glycoproteins of herpes simplex virus type 1: two forms of a single polypeptide.

Authors:  R Eberle; R J Courtney
Journal:  J Virol       Date:  1980-12       Impact factor: 5.103

3.  Characterization of herpesvirus sylvilagus glycoproteins released into the culture medium of infected cells: antisera to gp13 and gp32 neutralize viral infectivity in vitro and identify antigens on plasma membranes of infected cells.

Authors:  A K Patick; H C Hinze
Journal:  J Virol       Date:  1987-11       Impact factor: 5.103

4.  Characterization of the 92,000-dalton glycoprotein induced by herpes simplex virus type 2.

Authors:  H S Marsden; A Buckmaster; J W Palfreyman; R G Hope; A C Minson
Journal:  J Virol       Date:  1984-05       Impact factor: 5.103

5.  Requirements for transport of HSV-1 glycoproteins to the cell surface membrane of human fibroblasts and Vero cells.

Authors:  B Norrild; I Virtanen; B Pedersen; L Pereira
Journal:  Arch Virol       Date:  1983       Impact factor: 2.574

6.  Mapping and sequence of the gene for the pseudorabies virus glycoprotein which accumulates in the medium of infected cells.

Authors:  T J Rea; J G Timmins; G W Long; L E Post
Journal:  J Virol       Date:  1985-04       Impact factor: 5.103

7.  Antigenic variants of herpes simplex virus selected with glycoprotein-specific monoclonal antibodies.

Authors:  T C Holland; S D Marlin; M Levine; J Glorioso
Journal:  J Virol       Date:  1983-02       Impact factor: 5.103

8.  Crossed immunoelectrophoretic analysis of herpes simplex virus type 2 proteins. Characterization of antigen-5.

Authors:  B Norrild; A B Andersen; R Feldborg
Journal:  Arch Virol       Date:  1985       Impact factor: 2.574

9.  Immunological reactivity of herpes simplex virus 1 and 2 polypeptides electrophoretically separated and transferred to diazobenzyloxymethyl paper.

Authors:  B Norrild; B Pedersen; B Roizman
Journal:  Infect Immun       Date:  1981-02       Impact factor: 3.441
  9 in total

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