Literature DB >> 8556962

Diagnostic DNA amplification from individual tick eggs, larvae and nymphs.

M Zahler1, R Gothe, H Rinder.   

Abstract

The species of single tick eggs, larvae and nymphs was determined by PCR amplification and characterization of the hypervariable, second transcribed spacer (ITS2) of the multicopy ribosomal RNA gene (rDNA). Engorgement of larvae and nymphs did not preclude species identification. The method is generally applicable for ixodid and argasid ticks and can be used for epidemiological studies requiring the identification of individuals from pre-adult stages.

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Year:  1995        PMID: 8556962     DOI: 10.1007/bf00052084

Source DB:  PubMed          Journal:  Exp Appl Acarol        ISSN: 0168-8162            Impact factor:   2.132


  7 in total

Review 1.  Ribosomal DNA: molecular evolution and phylogenetic inference.

Authors:  D M Hillis; M T Dixon
Journal:  Q Rev Biol       Date:  1991-12       Impact factor: 4.875

2.  A rapid and complete 4-step protocol for obtaining nucleotide sequence from E. coli genomic DNA from overnight cultures.

Authors:  S Seetharam; I B Dicker
Journal:  Biotechniques       Date:  1991-07       Impact factor: 1.993

3.  Molecular taxonomy using single-strand conformation polymorphism (SSCP) analysis of mitochondrial ribosomal DNA genes.

Authors:  R H Hiss; D E Norris; C H Dietrich; R F Whitcomb; D F West; C F Bosio; S Kambhampati; J Piesman; M F Antolin; W C Black
Journal:  Insect Mol Biol       Date:  1994-08       Impact factor: 3.585

4.  Phylogeny of hard- and soft-tick taxa (Acari: Ixodida) based on mitochondrial 16S rDNA sequences.

Authors:  W C Black; J Piesman
Journal:  Proc Natl Acad Sci U S A       Date:  1994-10-11       Impact factor: 11.205

5.  Genetic evidence against a morphologically suggestive conspecificity of Dermacentor reticulatus and D. marginatus (Acari:Ixodidae).

Authors:  M Zahler; R Gothe; H Rinder
Journal:  Int J Parasitol       Date:  1995-12       Impact factor: 3.981

6.  Variation in ribosomal DNA internal transcribed spacers 1 among eastern populations of Ixodes scapularis (Acari: Ixodidae).

Authors:  D K McLain; D M Wesson; J H Oliver; F H Collins
Journal:  J Med Entomol       Date:  1995-05       Impact factor: 2.278

7.  Investigation of the validity of species status of Ixodes dammini (Acari: Ixodidae) using rDNA.

Authors:  D M Wesson; D K McLain; J H Oliver; J Piesman; F H Collins
Journal:  Proc Natl Acad Sci U S A       Date:  1993-11-01       Impact factor: 11.205

  7 in total
  5 in total

Review 1.  Molecular detection of pathogen DNA in ticks (Acari: Ixodidae): a review.

Authors:  O A Sparagano; M T Allsopp; R A Mank; S G Rijpkema; J V Figueroa; F Jongejan
Journal:  Exp Appl Acarol       Date:  1999-12       Impact factor: 2.132

Review 2.  The application of molecular markers in the study of diversity in acarology: a review.

Authors:  M Navajas; B Fenton
Journal:  Exp Appl Acarol       Date:  2000       Impact factor: 2.132

3.  The diguanylate cyclase, Rrp1, regulates critical steps in the enzootic cycle of the Lyme disease spirochetes.

Authors:  Jessica L Kostick; Lee T Szkotnicki; Elizabeth A Rogers; Paola Bocci; Nadia Raffaelli; Richard T Marconi
Journal:  Mol Microbiol       Date:  2011-06-05       Impact factor: 3.501

4.  Crossbreeding between different geographical populations of the brown dog tick, Rhipicephalus sanguineus (Acari: Ixodidae).

Authors:  M L Levin; E Studer; L Killmaster; G Zemtsova; K Y Mumcuoglu
Journal:  Exp Appl Acarol       Date:  2012-04-21       Impact factor: 2.132

5.  The Borrelia burgdorferi c-di-GMP Binding Receptors, PlzA and PlzB, Are Functionally Distinct.

Authors:  Jessica L Kostick-Dunn; Jerilyn R Izac; John C Freedman; Lee T Szkotnicki; Lee D Oliver; Richard T Marconi
Journal:  Front Cell Infect Microbiol       Date:  2018-07-11       Impact factor: 5.293

  5 in total

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