| Literature DB >> 8556493 |
Abstract
In this study, we demonstrated by a Cu-Zn superoxide dismutase-specific enzyme-linked immunoassay that cattle that are serologically positive for Brucella abortus have serum immunoglobulin G antibodies to B. abortus Cu-Zn superoxide dismutase. The specificity of the antibody reactivity was confirmed by Western blot (immunoblot) analysis with B. abortus salt-extractable proteins containing native Cu-Zn superoxide dismutase and with recombinant B. abortus Cu-Zn superoxide dismutase. The results represent a first step in the direction of the development of a multiprotein diagnostic reagent for bovine brucellosis.Entities:
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Year: 1994 PMID: 8556493 PMCID: PMC368323 DOI: 10.1128/cdli.1.5.506-510.1994
Source DB: PubMed Journal: Clin Diagn Lab Immunol ISSN: 1071-412X