| Literature DB >> 8555114 |
N Iler1, D H Rowitch, Y Echelard, A P McMahon, C Abate-Shen.
Abstract
In this study we investigate the molecular mechanisms that are responsible for the restricted expression of Wnt-1 during embryogenesis. We report that a single homeodomain binding site, HBS1, within the Wnt-1 enhancer contributes to appropriate spatial expression of Wnt-1 in the developing nervous system. This HBS1 site may be required for repressing Wnt-1 expression in the developing forebrain since specific mutations of this site result in an extension of the rostral boundary of Wnt-1/lacZ staining in transgenic embryos. We further demonstrate that a subset of homeodomain proteins expressed in the forebrain (i.e., Dix2, Emx2) interact specifically with HBS1. These findings suggest that these (or related) homeodomain proteins may regulate expression of Wnt-1 during normal brain development by interacting with the HBS1 site in the Wnt-1 enhancer.Entities:
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Year: 1995 PMID： 8555114 DOI： 10.1016/0925-4773(95)00427-0
Source DB: PubMed Journal: Mech Dev ISSN： 0925-4773 Impact factor: 1.882