Literature DB >> 8554764

Development of RAPD protocol for typing of strains of lactic acid bacteria and enterococci.

P S Cocconcelli1, D Porro, S Galandini, L Senini.   

Abstract

A protocol for typing strains of lactic acid bacteria and enterococci based on randomly amplified polymorphic DNA (RAPD) fragments has been developed. Using a single 10-mer primer, fingerprints were achieved without the need to isolate genomic DNA. Different conditions of DNA release and amplification were investigated in order to obtain reproducible results and high discrimination among strains. This RAPD protocol was successfully applied for the typing of strains belonging to the species Lactobacillus acidophilus, Lact. helveticus, Lact. casei, Lact. reuteri, Lact. plantarum, Enterococcus faecalis, Ent. faecium and Streptococcus thermophilus.

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Year:  1995        PMID： 8554764     DOI： 10.1111/j.1472-765x.1995.tb01085.x

Source DB:  PubMed          Journal:  Lett Appl Microbiol        ISSN： 0266-8254            Impact factor:   2.858


  18 in total

1.  Characterization of acetic acid bacteria in traditional acetic acid fermentation of rice vinegar (komesu) and unpolished rice vinegar (kurosu) produced in Japan.

Authors:  K Nanda; M Taniguchi; S Ujike; N Ishihara; H Mori; H Ono; Y Murooka
Journal:  Appl Environ Microbiol       Date:  2001-02       Impact factor: 4.792

2.  Analysis of three marine fish cell lines by rapd assay.

Authors:  H R Guo; S C Zhang; S L Tong; J H Xiang
Journal:  In Vitro Cell Dev Biol Anim       Date:  2001 Jul-Aug       Impact factor: 2.416

3.  Growth and consumption of L-malic acid in wine-like medium by acclimated and non-acclimated cultures of Patagonian Oenococcus oeni strains.

Authors:  Bárbara Mercedes Bravo-Ferrada; Axel Hollmann; Natalia Brizuela; Danay Valdés La Hens; Elizabeth Tymczyszyn; Liliana Semorile
Journal:  Folia Microbiol (Praha)       Date:  2016-01-23       Impact factor: 2.099

4.  Molecular typing of selected Enterococcus faecalis isolates: pilot study using multilocus sequence typing and pulsed-field gel electrophoresis.

Authors:  Sreedhar R Nallapareddy; Ruay-Wang Duh; Kavindra V Singh; Barbara E Murray
Journal:  J Clin Microbiol       Date:  2002-03       Impact factor: 5.948

5.  RAPD-PCR analysis for molecular characterization and genotoxic studies of a new marine fish cell line derived from Dicentrarchus labrax.

Authors:  L Rocco; I V Valentino; G Scapigliati; V Stingo
Journal:  Cytotechnology       Date:  2013-07-10       Impact factor: 2.058

Review 6.  Patagonian red wines: selection of Lactobacillus plantarum isolates as potential starter cultures for malolactic fermentation.

Authors:  Bárbara Mercedes Bravo-Ferrada; Axel Hollmann; Lucrecia Delfederico; Danay Valdés La Hens; Adriana Caballero; Liliana Semorile
Journal:  World J Microbiol Biotechnol       Date:  2013-04-02       Impact factor: 3.312

7.  Random Amplified Polymorphic DNA (RAPD) Typing of Multidrug Resistant Enterococcus faecium Urinary Isolates from a Tertiary Care Centre, Northern India.

Authors:  Tuhina Banerjee
Journal:  J Clin Diagn Res       Date:  2013-12-15

8.  Strain-specific identification of probiotic Lactobacillus rhamnosus with randomly amplified polymorphic DNA-derived PCR primers.

Authors:  A Tilsala-Timisjärvi; T Alatossava
Journal:  Appl Environ Microbiol       Date:  1998-12       Impact factor: 4.792

9.  An ecological study of lactococci isolated from raw milk in the camembert cheese registered designation of origin area.

Authors:  D Corroler; I Mangin; N Desmasures; M Gueguen
Journal:  Appl Environ Microbiol       Date:  1998-12       Impact factor: 4.792

10.  Differentiation of dextran-producing leuconostoc strains by a modified randomly amplified polymorphic DNA protocol

Authors: 
Journal:  Appl Environ Microbiol       Date:  1998-08       Impact factor: 4.792
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