Literature DB >> 8553694

DOGR1 and DOGR2: two genes from Saccharomyces cerevisiae that confer 2-deoxyglucose resistance when overexpressed.

F Randez-Gil1, A Blasco, J A Prieto, P Sanz.   

Abstract

Saccharomyces cerevisiae contains two genes (DOGR1 and DOGR2) that are able to confer 2-deoxyglucose resistance when they are overexpressed. These genes are very similar, sharing 92% identity at the protein level. They code for two isoenzymes with 2-deoxyglucose-6 phosphate (2-DOG-6P) phosphatase activity. These enzymes have been purified and characterized. DogR1p shows an optimum pH of 6, an optimum temperature of 30 degrees C and a KM on 2-DOG-6P of 17 mM. DogR2p shows a similar optimum pH, but the optimum temperature is 40 degrees C and it exhibits a KM on 2-DOG-6P of 41 mM. Both enzymes require 10 mM-MgCl2 for maximal activity and they are inhibited by inorganic phosphate.

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Year:  1995        PMID： 8553694     DOI： 10.1002/yea.320111303

Source DB:  PubMed          Journal:  Yeast        ISSN： 0749-503X            Impact factor:   3.239


  15 in total

1.  Improved properties of baker's yeast mutants resistant to 2-deoxy-D-glucose.

Authors:  A M Rincón; A C Codón; F Castrejón; T Benítez
Journal:  Appl Environ Microbiol       Date:  2001-09       Impact factor: 4.792

2.  Cooperative regulation of DOG2, encoding 2-deoxyglucose-6-phosphate phosphatase, by Snf1 kinase and the high-osmolarity glycerol-mitogen-activated protein kinase cascade in stress responses of Saccharomyces cerevisiae.

Authors:  Y Tsujimoto; S Izawa; Y Inoue
Journal:  J Bacteriol       Date:  2000-09       Impact factor: 3.490

3.  The Small Protein SgrT Controls Transport Activity of the Glucose-Specific Phosphotransferase System.

Authors:  Chelsea R Lloyd; Seongjin Park; Jingyi Fei; Carin K Vanderpool
Journal:  J Bacteriol       Date:  2017-05-09       Impact factor: 3.490

4.  Functional Diversity of Haloacid Dehalogenase Superfamily Phosphatases from Saccharomyces cerevisiae: BIOCHEMICAL, STRUCTURAL, AND EVOLUTIONARY INSIGHTS.

Authors:  Ekaterina Kuznetsova; Boguslaw Nocek; Greg Brown; Kira S Makarova; Robert Flick; Yuri I Wolf; Anna Khusnutdinova; Elena Evdokimova; Ke Jin; Kemin Tan; Andrew D Hanson; Ghulam Hasnain; Rémi Zallot; Valérie de Crécy-Lagard; Mohan Babu; Alexei Savchenko; Andrzej Joachimiak; Aled M Edwards; Eugene V Koonin; Alexander F Yakunin
Journal:  J Biol Chem       Date:  2015-06-12       Impact factor: 5.157

5.  Arabidopsis thaliana AtGppl and AtGpp2: two novel low molecular weight phosphatases involved in plant glycerol metabolism.

Authors:  José Antonio Caparrós-Martín; Sonja Reiland; Karl Köchert; Mari Cruz Cutanda; Francisco A Culiáñez-Macià
Journal:  Plant Mol Biol       Date:  2007-03       Impact factor: 4.076

6.  2-Deoxyglucose impairs Saccharomyces cerevisiae growth by stimulating Snf1-regulated and α-arrestin-mediated trafficking of hexose transporters 1 and 3.

Authors:  Allyson F O'Donnell; Rhonda R McCartney; Dakshayini G Chandrashekarappa; Bob B Zhang; Jeremy Thorner; Martin C Schmidt
Journal:  Mol Cell Biol       Date:  2014-12-29       Impact factor: 4.272

7.  Mass spectrometry-based metabolomics of single yeast cells.

Authors:  Alfredo J Ibáñez; Stephan R Fagerer; Anna Mareike Schmidt; Pawel L Urban; Konstantins Jefimovs; Philipp Geiger; Reinhard Dechant; Matthias Heinemann; Renato Zenobi
Journal:  Proc Natl Acad Sci U S A       Date:  2013-05-13       Impact factor: 11.205

8.  The kinetic analysis of the substrate specificity of motif 5 in a HAD hydrolase-type phosphosugar phosphatase of Arabidopsis thaliana.

Authors:  José A Caparrós-Martín; Iva McCarthy-Suárez; Francisco A Culiáñez-Macià
Journal:  Planta       Date:  2014-06-11       Impact factor: 4.116

9.  Metabolic engineering of Saccharomyces cerevisiae for conversion of D-glucose to xylitol and other five-carbon sugars and sugar alcohols.

Authors:  Mervi H Toivari; Laura Ruohonen; Andrei N Miasnikov; Peter Richard; Merja Penttilä
Journal:  Appl Environ Microbiol       Date:  2007-07-13       Impact factor: 4.792

10.  HAD hydrolase function unveiled by substrate screening: enzymatic characterization of Arabidopsis thaliana subclass I phosphosugar phosphatase AtSgpp.

Authors:  José A Caparrós-Martín; Iva McCarthy-Suárez; Francisco A Culiáñez-Macià
Journal:  Planta       Date:  2012-11-24       Impact factor: 4.116
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