Literature DB >> 8550589

Conformational changes in oxyhemoglobin C (Glu beta 6-->Lys) detected by spectroscopic probing.

R E Hirsch1, M J Lin, G J Vidugiris, S Huang, J M Friedman, R L Nagel, G V Vidugirus.   

Abstract

Hemoglobin C (Glu beta 6-->Lys) shares with hemoglobin S (Glu beta 6-->Val) the site of mutation, but with different consequences: deoxyHbS forms polymers, whereas oxyHbC readily forms crystals. The molecular mechanism for this property of oxyHbC is unknown. Since no detailed oxyHbC crystal structural information exists, spectroscopic probing is used in this study to investigate possible solution-phase conformational changes in HbC compared with HbA. Intrinsic fluorescence combined with UV resonance Raman data demonstrate a weakening of the Trp beta 15-Ser beta 72 hydrogen bond that most likely leads to a displacement of the A helix away from the E helix.

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Year:  1996        PMID： 8550589     DOI： 10.1074/jbc.271.1.372

Source DB:  PubMed          Journal:  J Biol Chem        ISSN： 0021-9258            Impact factor:   5.157


  5 in total

1.  Intermolecular interactions, nucleation, and thermodynamics of crystallization of hemoglobin C.

Authors:  Peter G Vekilov; Angela R Feeling-Taylor; Dimiter N Petsev; Oleg Galkin; Ronald L Nagel; Rhoda Elison Hirsch
Journal:  Biophys J       Date:  2002-08       Impact factor: 4.033

2.  Liquid-liquid phase separation in hemoglobins: distinct aggregation mechanisms of the beta6 mutants.

Authors:  Qiuying Chen; Peter G Vekilov; Ronald L Nagel; Rhoda Elison Hirsch
Journal:  Biophys J       Date:  2004-03       Impact factor: 4.033

3.  Structural and functional studies indicating altered redox properties of hemoglobin E: implications for production of bioactive nitric oxide.

Authors:  Camille J Roche; Vladimir Malashkevich; Tatiana C Balazs; David Dantsker; Qiuying Chen; Juan Moreira; Steven C Almo; Joel M Friedman; Rhoda Elison Hirsch
Journal:  J Biol Chem       Date:  2011-04-29       Impact factor: 5.157

4.  Liganded hemoglobin structural perturbations by the allosteric effector L35.

Authors:  Qiuying Chen; Iraj Lalezari; Ronald L Nagel; Rhoda Elison Hirsch
Journal:  Biophys J       Date:  2004-12-30       Impact factor: 4.033

5.  Raman spectroscopy for a rapid diagnosis of sickle cell disease in human blood samples: a preliminary study.

Authors:  Antonio Carlos Bueno Filho; Landulfo Silveira; Ana Leticia Sant'Anna Yanai; Adriana Barrinha Fernandes
Journal:  Lasers Med Sci       Date:  2014-09-13       Impact factor: 3.161
  5 in total

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