Literature DB >> 8550434

Cloning and overexpression in Escherichia coli of the genes encoding NAD-dependent alcohol dehydrogenase from two Sulfolobus species.

R Cannio1, G Fiorentino, P Carpinelli, M Rossi, S Bartolucci.   

Abstract

The gene adh encoding a NAD-dependent alcohol dehydrogenase from the novel strain RC3 of Sulfolobus sp. was cloned and sequenced. Both the adh gene from Sulfolobus sp. strain RC3 and the alcohol dehydrogenase gene from Sulfolobus solfataricus (DSM 1617) were expressed at a high level in Escherichia coli, and the recombinant enzymes were purified, characterized, and compared. Only a few amino acid replacements were responsible for the different kinetic and physicochemical features investigated.

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Year:  1996        PMID： 8550434      PMCID： PMC177655          DOI： 10.1128/jb.178.1.301-305.1996

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  13 in total

Review 1.  Hyperthermophilic enzymes: sources, uses, and molecular mechanisms for thermostability.

Authors:  C Vieille; G J Zeikus
Journal:  Microbiol Mol Biol Rev       Date:  2001-03       Impact factor: 11.056

2.  Characterization of alcohol dehydrogenase (ADH12) from Haloarcula marismortui, an extreme halophile from the Dead Sea.

Authors:  Leanne M Timpson; Diya Alsafadi; Cillín Mac Donnchadha; Susan Liddell; Michael A Sharkey; Francesca Paradisi
Journal:  Extremophiles       Date:  2011-10-21       Impact factor: 2.395

3.  MarR-like transcriptional regulator involved in detoxification of aromatic compounds in Sulfolobus solfataricus.

Authors:  Gabriella Fiorentino; Raffaele Ronca; Raffaele Cannio; Mosè Rossi; Simonetta Bartolucci
Journal:  J Bacteriol       Date:  2007-08-03       Impact factor: 3.490

4.  Selective recruitment of Adh genes for distinct enzymatic functions in Petunia hybrida.

Authors:  Freydoun Garabagi; Gregory Duns; Judith Strommer
Journal:  Plant Mol Biol       Date:  2005-05       Impact factor: 4.076

5.  Heterologous expression and characterization of an alcohol dehydrogenase from the archeon Thermoplasma acidophilum.

Authors:  Erika Nahomy Marino-Marmolejo; Antonio De León-Rodríguez; Ana Paulina Barba de la Rosa; Leticia Santos
Journal:  Mol Biotechnol       Date:  2008-12-05       Impact factor: 2.695

Review 6.  Host and viral transcriptional regulators in Sulfolobus: an overview.

Authors:  Patrizia Contursi; Salvatore Fusco; Danila Limauro; Gabriella Fiorentino
Journal:  Extremophiles       Date:  2013-10-02       Impact factor: 2.395

7.  Sulfolobus tokodaii ST0053 produces a novel thermostable, NAD-dependent medium-chain alcohol dehydrogenase.

Authors:  Hisaaki Yanai; Katsumi Doi; Toshihisa Ohshima
Journal:  Appl Environ Microbiol       Date:  2009-01-09       Impact factor: 4.792

8.  Transcriptional regulation of the gene encoding an alcohol dehydrogenase in the archaeon Sulfolobus solfataricus involves multiple factors and control elements.

Authors:  Gabriella Fiorentino; Raffaele Cannio; Mosè Rossi; Simonetta Bartolucci
Journal:  J Bacteriol       Date:  2003-07       Impact factor: 3.490

9.  Influence of temperature on the production of an archaeal thermoactive alcohol dehydrogenase from Pyrococcus furiosus with recombinant Escherichia coli.

Authors:  Jürgen Kube; Christian Brokamp; Ronnie Machielsen; John van der Oost; Herbert Märkl
Journal:  Extremophiles       Date:  2006-02-07       Impact factor: 2.395

10.  Molecular characterization of the recombinant iron-containing alcohol dehydrogenase from the hyperthermophilic Archaeon, Thermococcus strain ES1.

Authors:  Xiangxian Ying; Amy M Grunden; Lin Nie; Michael W W Adams; Kesen Ma
Journal:  Extremophiles       Date:  2008-12-25       Impact factor: 2.395
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