Literature DB >> 8549830

Schizosaccharomyces pombe possesses an unusual and a conventional hexokinase: biochemical and molecular characterization of both hexokinases.

T Petit1, M A Blázquez, C Gancedo.   

Abstract

Two hexokinases were characterized in Schizosaccharomyces pombe: hexokinase 1, with a low phosphorylation coefficient on glucose (Km 8.5 mM) and hexokinase 2, a kinetically conventional hexokinase. Genes hxk1+ and hxk2+ encoding these enzymes were cloned and sequenced. Disruption of hxk1+ had no effect on growth but disruption of hxk2+ doubled the generation time in glucose. Spores carrying the double disruption hxk1+ hxk2+ did not grow on glucose or fructose after one week. Expression of hxk1+ increased strongly during growth in fructose or glycerol. Expression of hxk2+ was highest during growth in glycerol. A NADP-dependent glucose dehydrogenase was detected, but not a glucokinase.

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Year:  1996        PMID： 8549830     DOI： 10.1016/0014-5793(95)01451-9

Source DB:  PubMed          Journal:  FEBS Lett        ISSN： 0014-5793            Impact factor:   4.124


  9 in total

1.  Isolation and characterization of Nrf1p, a novel negative regulator of the Cdc42p GTPase in Schizosaccharomyces pombe.

Authors:  J M Murray; D I Johnson
Journal:  Genetics       Date:  2000-01       Impact factor: 4.562

2.  The Aspergillus nidulans xprF gene encodes a hexokinase-like protein involved in the regulation of extracellular proteases.

Authors:  M E Katz; A Masoumi; S R Burrows; C G Shirtliff; B F Cheetham
Journal:  Genetics       Date:  2000-12       Impact factor: 4.562

3.  Multiple hexose transporters of Schizosaccharomyces pombe.

Authors:  S Heiland; N Radovanovic; M Höfer; J Winderickx; H Lichtenberg
Journal:  J Bacteriol       Date:  2000-04       Impact factor: 3.490

4.  Hexokinase regulates kinetics of glucose transport and expression of genes encoding hexose transporters in Saccharomyces cerevisiae.

Authors:  T Petit; J A Diderich; A L Kruckeberg; C Gancedo; K Van Dam
Journal:  J Bacteriol       Date:  2000-12       Impact factor: 3.490

Review 5.  Involvement of Dcr1 in post-transcriptional regulation of gene expression in Schizosaccharomyces pombe.

Authors:  Lise-Andree Gobeil; Pierre Plante; Mina Rohani; Marc Ouellette; Patrick Provost
Journal:  Front Biosci       Date:  2008-01-01

6.  Genome-wide characterization of the phosphate starvation response in Schizosaccharomyces pombe.

Authors:  Ian Carter-O'Connell; Michael T Peel; Dennis D Wykoff; Erin K O'Shea
Journal:  BMC Genomics       Date:  2012-12-12       Impact factor: 3.969

7.  Disruption of Yarrowia lipolytica TPS1 gene encoding trehalose-6-P synthase does not affect growth in glucose but impairs growth at high temperature.

Authors:  Carmen-Lisset Flores; Carlos Gancedo; Thomas Petit
Journal:  PLoS One       Date:  2011-09-12       Impact factor: 3.240

8.  The gene YALI0E20207g from Yarrowia lipolytica encodes an N-acetylglucosamine kinase implicated in the regulated expression of the genes from the N-acetylglucosamine assimilatory pathway.

Authors:  Carmen-Lisset Flores; Carlos Gancedo
Journal:  PLoS One       Date:  2015-03-27       Impact factor: 3.240

9.  Pro-aging effects of glucose signaling through a G protein-coupled glucose receptor in fission yeast.

Authors:  Antoine E Roux; Alexandre Leroux; Manal A Alaamery; Charles S Hoffman; Pascal Chartrand; Gerardo Ferbeyre; Luis A Rokeach
Journal:  PLoS Genet       Date:  2009-03-06       Impact factor: 5.917
  9 in total

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