Literature DB >> 8548879

Investigations on the mechanisms of methotrexate resistance in a cisplatin-resistant L1210 murine leukemia cell subline.

D H Wroblewski1, A Bhushan, Y Xuan, B T Brinton, T R Tritton, M P Hacker.   

Abstract

We report a murine leukemia cell variant (L1210/DDP), selected for cisplatin (DDP) resistance, to be cross-resistant to methotrexate (MTX). Cross-resistance of L1210 cells to DDP and MTX has been observed by others, and has also been recorded in P388 murine leukemia and SSC-25 human squamous carcinoma cells. We demonstrated that MTX resistance is not due to dihydrofolate reductase (DHFR) gene amplification, increased DHFR enzyme activity or decreased MTX binding to the target enzyme. Of the mechanisms commonly proposed for MTX resistance, only differences in transport were observed when comparing sensitive (L1210/0) and resistant (L1210/DDP) cells. Our results suggest that MTX resistance in L1210/DDP cells is due to altered methotrexate uptake.

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Year:  1996        PMID： 8548879     DOI： 10.1007/s002800050394

Source DB:  PubMed          Journal:  Cancer Chemother Pharmacol        ISSN： 0344-5704            Impact factor:   3.333


  2 in total

1.  Tyrosine phosphorylation of HSC70 and its interaction with RFC mediates methotrexate resistance in murine L1210 leukemia cells.

Authors:  Tuoen Liu; Ratan Singh; Zechary Rios; Alok Bhushan; Mengxiong Li; Peter P Sheridan; Shawn E Bearden; James C K Lai; Senyo Agbenowu; Shousong Cao; Christopher K Daniels
Journal:  Cancer Lett       Date:  2014-11-20       Impact factor: 8.679

2.  Identification and characterization of a 66-68-kDa protein as a methotrexate-binding protein in murine leukemia L1210 cells.

Authors:  Tuoen Liu; Allison Dean; Saint Ashwini; Peter P Sheridan; Alok Bhushan; James C K Lai; Shousong Cao; Christopher K Daniels
Journal:  Cell Stress Chaperones       Date:  2012-10-23       Impact factor: 3.667
  2 in total

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