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Literature DB >> 8548530

Monoclonal antibodies to Coxiella burnetii that cross-react with strain Nine Mile.

Z Sekeyová¹, E Kovácová, J Kazár, R Toman, S Olvecká.

Abstract

Results are presented to show the binding properties of five monoclonal antibodies directed to Coxiella burnetii Priscilla with cross-reactions to the Nine Mile strain. The monoclonal antibodies preferentially recognize phase I epitopes by ELISA and recognize phase II epitopes by immunoblotting but do not allow differentiation between so-called chronic and acute strains of C. burnetii. The only difference in reactivity was in the staining pattern revealed after reactions with lipopolysaccharide I antigens.

Entities: Chemical Disease Species

Mesh：

Substances：

Year: 1995 PMID： 8548530 PMCID： PMC170195 DOI： 10.1128/cdli.2.5.531-534.1995

Source DB: PubMed Journal: Clin Diagn Lab Immunol ISSN： 1071-412X

25 in total

1. A method of purifying Coxiella burnetii and other pathogenic Rickettsiae.

Authors: R A ORMSBEE
Journal: J Immunol Date: 1962-01 Impact factor: 5.422

2. Reactivity of monoclonal antibodies to Rickettsia rickettsii with spotted fever and typhus group rickettsiae.

Authors: R L Anacker; R E Mann; C Gonzales
Journal: J Clin Microbiol Date: 1987-01 Impact factor: 5.948

3. Neutralizing activity of monoclonal antibodies to heat-sensitive and heat-resistant epitopes of Rickettsia rickettsii surface proteins.

Authors: R L Anacker; G A McDonald; R H List; R E Mann
Journal: Infect Immun Date: 1987-03 Impact factor: 3.441

4. The analysis of lipopolysaccharide (endotoxin) in meningococcal polysaccharide vaccines by silver staining following SDS-polyacrylamide gel electrophoresis.

Authors: C M Tsai
Journal: J Biol Stand Date: 1986-01

Monoclonal antibodies to Coxiella burnetii that cross-react with strain Nine Mile.

1. A method of purifying Coxiella burnetii and other pathogenic Rickettsiae.

2. Reactivity of monoclonal antibodies to Rickettsia rickettsii with spotted fever and typhus group rickettsiae.

3. Neutralizing activity of monoclonal antibodies to heat-sensitive and heat-resistant epitopes of Rickettsia rickettsii surface proteins.

4. The analysis of lipopolysaccharide (endotoxin) in meningococcal polysaccharide vaccines by silver staining following SDS-polyacrylamide gel electrophoresis.

5. Production and characterization of cloned T-cell hybridomas that are responsive to Rickettsia conorii antigens.

6. The effect of postfusion cell density on establishment of hybridomas.

7. Coxiella burnetii antigens for detection of Q fever antibodies by ELISA in human sera.

8. Preparation and characterization of hybridomas producing monoclonal antibodies against human alpha interferon.

9. Monoclonal antibodies distinguish phase variants of Coxiella burnetii.

10. Antigenic variation in the phase I lipopolysaccharide of Coxiella burnetii isolates.

1. Proteomics paves the way for Q fever diagnostics.