Literature DB >> 8546455

Sizing of the Rhodococcus sp. R312 genome by pulsed-field gel electrophoresis. Localization of genes involved in nitrile degradation.

F Bigey1, G Janbon, A Arnaud, P Galzy.   

Abstract

The two restriction enzymes AsnI and DraI were found to produce DNA fragment sizes that could be used for mapping the Rhodococcus sp. R312 (formerly Brevibacterium sp. R312) genome by pulsed-field gel electrophoresis. AsnI produced 24 fragments (4 to 727 kb) and DraI yielded 15 fragments (8.5 to 2400 kb). The fragment lengths in each digest were summed, indicating that the size of the chromosome ranged from 6.31 to 6.56 Mb, with a mean of 6.44 Mb. In addition, the wide-spectrum amidase gene (amiE) and the operon containing the enantiomer-selective amidase gene (amdA) and the nitrile hydratase structural gene (nthA, nthB) were localized on the AsnI and DraI fragments.

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Year:  1995        PMID： 8546455     DOI： 10.1007/bf00873103

Source DB:  PubMed          Journal:  Antonie Van Leeuwenhoek        ISSN： 0003-6072            Impact factor:   2.271


  12 in total

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Authors:  M Kobayashi; T Nagasawa; H Yamada
Journal:  Trends Biotechnol       Date:  1992-11       Impact factor: 19.536

2.  Restriction endonucleases for pulsed field mapping of bacterial genomes.

Authors:  M McClelland; R Jones; Y Patel; M Nelson
Journal:  Nucleic Acids Res       Date:  1987-08-11       Impact factor: 16.971

3.  Cloning and primary structure of the wide-spectrum amidase from Brevibacterium sp. R312: high homology to the amiE product from Pseudomonas aeruginosa.

Authors:  F Soubrier; S Lévy-Schil; J F Mayaux; D Pétré; A Arnaud; J Crouzet
Journal:  Gene       Date:  1992-07-01       Impact factor: 3.688

4.  Rapid determnation of DNA base composition by ultraviolet spectroscopy.

Authors:  S Ulitzur
Journal:  Biochim Biophys Acta       Date:  1972-06-22

5.  Separation of large DNA molecules by contour-clamped homogeneous electric fields.

Authors:  G Chu; D Vollrath; R W Davis
Journal:  Science       Date:  1986-12-19       Impact factor: 47.728

6.  Purification, cloning, and primary structure of an enantiomer-selective amidase from Brevibacterium sp. strain R312: structural evidence for genetic coupling with nitrile hydratase.

Authors:  J F Mayaux; E Cerebelaud; F Soubrier; D Faucher; D Pétré
Journal:  J Bacteriol       Date:  1990-12       Impact factor: 3.490

7.  Pulsed-field gel electrophoresis analysis of the genome of Streptomyces ambofaciens strains.

Authors:  P Leblond; F X Francou; J M Simonet; B Decaris
Journal:  FEMS Microbiol Lett       Date:  1990-10       Impact factor: 2.742

8.  Separation of yeast chromosome-sized DNAs by pulsed field gradient gel electrophoresis.

Authors:  D C Schwartz; C R Cantor
Journal:  Cell       Date:  1984-05       Impact factor: 41.582

9.  Identification and characterization of a transmissible linear plasmid from Rhodococcus erythropolis BD2 that encodes isopropylbenzene and trichloroethene catabolism.

Authors:  B Dabrock; M Kesseler; B Averhoff; G Gottschalk
Journal:  Appl Environ Microbiol       Date:  1994-03       Impact factor: 4.792

Review 10.  The biology and genetics of the genus Rhodococcus.

Authors:  W R Finnerty
Journal:  Annu Rev Microbiol       Date:  1992       Impact factor: 15.500
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  2 in total

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Authors:  Stéphane Uroz; Phil M Oger; Emilie Chapelle; Marie-Thérèse Adeline; Denis Faure; Yves Dessaux
Journal:  Appl Environ Microbiol       Date:  2008-01-11       Impact factor: 4.792

2.  The complete genome sequence of the nitrile biocatalyst Rhodocccus rhodochrous ATCC BAA-870.

Authors:  Joni Frederick; Fritha Hennessy; Uli Horn; Pilar de la Torre Cortés; Marcel van den Broek; Ulrich Strych; Richard Willson; Charles A Hefer; Jean-Marc G Daran; Trevor Sewell; Linda G Otten; Dean Brady
Journal:  BMC Genomics       Date:  2020-01-02       Impact factor: 3.969
  2 in total

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