| Literature DB >> 8544211 |
M Trautmann1, A S Cross, G Reich, H Held, R Podschun, R Marre.
Abstract
Strains of Klebsiella spp. are often inagglutinable by O-specific antisera because of the copious capsule produced by most isolates. A competitive ELISA method based on the observation that bacterial supernates containing homologous O antigen specifically inhibited the reaction of type-specific antisera with purified LPS coated on ELISA plates was used to examine the O antigen of 82 isolates of different Klebsiella species and subspecies. The O antigens O1/2ab (19 isolates), O2ab (13 isolates), O2ac (11 isolates) and O3 (16 isolates) were found to account for > 70% of the O antigenic types. Overall, 65 (79%) of the strains could be assigned to a specific O serogroup. The method is suitable for examining the role of individual O antigens in systemic klebsiella infections such as nosocomial septicaemia and pneumonia.Entities:
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Year: 1996 PMID： 8544211 DOI： 10.1099/00222615-44-1-44
Source DB: PubMed Journal: J Med Microbiol ISSN： 0022-2615 Impact factor: 2.472