BACKGROUND: Type I allergic symptoms and severe asthma in particular are frequently caused by animal hair/dander proteins, among which albumins are possible cross-sensitizing allergenic components. METHODS: The significance and degree of IgE-cross-reactivities against various albumins were studied in a representative number (n = 200) of patients allergic to animals with hair/dander extracts, purified albumins from different animals, and a recombinant dog albumin fragment expressed in lysogenic Escherichia coli Y1089 and purified as a beta-galactosidase fusion protein. RESULTS: Despite a high degree of sequence homology among different albumins, a remarkable variability of IgE cross-reactivities was observed, indicating that some patients were sensitized preferentially against certain albumins. Most of the patients allergic to albumins, however, reacted to dog, cat, and horse albumin, which also bound a high percentage of albumin-specific IgE. CONCLUSION: The purified recombinant dog albumin fragment, representing 265 amino acids of the mature protein, bound IgE from all 15 patients allergic to albumin tested suggesting its potential usefulness for diagnosis and perhaps therapy.
BACKGROUND:Type I allergic symptoms and severe asthma in particular are frequently caused by animal hair/dander proteins, among which albumins are possible cross-sensitizing allergenic components. METHODS: The significance and degree of IgE-cross-reactivities against various albumins were studied in a representative number (n = 200) of patientsallergic to animals with hair/dander extracts, purified albumins from different animals, and a recombinant dog albumin fragment expressed in lysogenic Escherichia coli Y1089 and purified as a beta-galactosidase fusion protein. RESULTS: Despite a high degree of sequence homology among different albumins, a remarkable variability of IgE cross-reactivities was observed, indicating that some patients were sensitized preferentially against certain albumins. Most of the patientsallergic to albumins, however, reacted to dog, cat, and horse albumin, which also bound a high percentage of albumin-specific IgE. CONCLUSION: The purified recombinant dog albumin fragment, representing 265 amino acids of the mature protein, bound IgE from all 15 patientsallergic to albumin tested suggesting its potential usefulness for diagnosis and perhaps therapy.
Authors: Maksymilian Chruszcz; Fook Tim Chew; Karin Hoffmann-Sommergruber; Barry K Hurlburt; Geoffrey A Mueller; Anna Pomés; Juha Rouvinen; Mayte Villalba; Birgitta M Wöhrl; Heimo Breiteneder Journal: Allergy Date: 2021-05-02 Impact factor: 14.710
Authors: Anna Krakowiak; Marta Wiszniewska; Patrycja Krawczyk; Bogdan Szulc; Tomasz Wittczak; Jolanta Walusiak; Cezary Pałczynski Journal: Int Arch Occup Environ Health Date: 2006-10-05 Impact factor: 2.851
Authors: Monika Raulf; K C Bergmann; S Kull; I Sander; Ch Hilger; T Brüning; U Jappe; H Müsken; A Sperl; S Vrtala; E Zahradnik; L Klimek Journal: Allergo J Int Date: 2015-05-09