Literature DB >> 8543054

Purification and reconstitution of activity of Saccharomyces cerevisiae P450 61, a sterol delta 22-desaturase.

S L Kelly1, D C Lamb, A J Corran, B C Baldwin, L W Parks, D E Kelly.   

Abstract

P450 was purified from microsomal fractions of a strain of Saccharomyces cerevisiae which contained detectable P450 despite the disruption of CYP51A1. The P450 had a molecular mass of 58 kDa, similar to P450 51A1, and in a reconstituted assay with rabbit NADPH-P450 reductase and dilauryl phosphotidylcholine exhibited activity for conversion of ergosta-5,7-dienol into ergosterol. N-Terminal amino acid sequencing of the purified protein corresponded to the translated sequence of P450 61 which was recently identified during sequencing of chromosome XIII. This allowed the function of this family of P450 to be identified as sterol delta 22-desaturation in the pathway of ergosterol biosynthesis.

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Year:  1995        PMID： 8543054     DOI： 10.1016/0014-5793(95)01342-3

Source DB:  PubMed          Journal:  FEBS Lett        ISSN： 0014-5793            Impact factor:   4.124


  14 in total

1.  Comparison of D0870, a new triazole antifungal agent, to fluconazole for inhibition of Candida albicans cytochrome P-450 by using in vitro assays.

Authors:  K Venkateswarlu; D W Denning; N J Manning; S L Kelly
Journal:  Antimicrob Agents Chemother       Date:  1996-06       Impact factor: 5.191

2.  Comparative genomics reveals high biological diversity and specific adaptations in the industrially and medically important fungal genus Aspergillus.

Authors:  Ronald P de Vries; Robert Riley; Ad Wiebenga; Guillermo Aguilar-Osorio; Sotiris Amillis; Cristiane Akemi Uchima; Gregor Anderluh; Mojtaba Asadollahi; Marion Askin; Kerrie Barry; Evy Battaglia; Özgür Bayram; Tiziano Benocci; Susanna A Braus-Stromeyer; Camila Caldana; David Cánovas; Gustavo C Cerqueira; Fusheng Chen; Wanping Chen; Cindy Choi; Alicia Clum; Renato Augusto Corrêa Dos Santos; André Ricardo de Lima Damásio; George Diallinas; Tamás Emri; Erzsébet Fekete; Michel Flipphi; Susanne Freyberg; Antonia Gallo; Christos Gournas; Rob Habgood; Matthieu Hainaut; María Laura Harispe; Bernard Henrissat; Kristiina S Hildén; Ryan Hope; Abeer Hossain; Eugenia Karabika; Levente Karaffa; Zsolt Karányi; Nada Kraševec; Alan Kuo; Harald Kusch; Kurt LaButti; Ellen L Lagendijk; Alla Lapidus; Anthony Levasseur; Erika Lindquist; Anna Lipzen; Antonio F Logrieco; Andrew MacCabe; Miia R Mäkelä; Iran Malavazi; Petter Melin; Vera Meyer; Natalia Mielnichuk; Márton Miskei; Ákos P Molnár; Giuseppina Mulé; Chew Yee Ngan; Margarita Orejas; Erzsébet Orosz; Jean Paul Ouedraogo; Karin M Overkamp; Hee-Soo Park; Giancarlo Perrone; Francois Piumi; Peter J Punt; Arthur F J Ram; Ana Ramón; Stefan Rauscher; Eric Record; Diego Mauricio Riaño-Pachón; Vincent Robert; Julian Röhrig; Roberto Ruller; Asaf Salamov; Nadhira S Salih; Rob A Samson; Erzsébet Sándor; Manuel Sanguinetti; Tabea Schütze; Kristina Sepčić; Ekaterina Shelest; Gavin Sherlock; Vicky Sophianopoulou; Fabio M Squina; Hui Sun; Antonia Susca; Richard B Todd; Adrian Tsang; Shiela E Unkles; Nathalie van de Wiele; Diana van Rossen-Uffink; Juliana Velasco de Castro Oliveira; Tammi C Vesth; Jaap Visser; Jae-Hyuk Yu; Miaomiao Zhou; Mikael R Andersen; David B Archer; Scott E Baker; Isabelle Benoit; Axel A Brakhage; Gerhard H Braus; Reinhard Fischer; Jens C Frisvad; Gustavo H Goldman; Jos Houbraken; Berl Oakley; István Pócsi; Claudio Scazzocchio; Bernhard Seiboth; Patricia A vanKuyk; Jennifer Wortman; Paul S Dyer; Igor V Grigoriev
Journal:  Genome Biol       Date:  2017-02-14       Impact factor: 13.583

Review 3.  Microbial cytochromes P450: biodiversity and biotechnology. Where do cytochromes P450 come from, what do they do and what can they do for us?

Authors:  Steven L Kelly; Diane E Kelly
Journal:  Philos Trans R Soc Lond B Biol Sci       Date:  2013-01-06       Impact factor: 6.237

4.  Phylogeny of Leptographium qinlingensis cytochrome P450 genes and transcription levels of six CYPs in response to different nutrition media or terpenoids.

Authors:  Lulu Dai; Jie Zheng; Jiaqi Ye; Hui Chen
Journal:  Arch Microbiol       Date:  2021-12-11       Impact factor: 2.552

5.  CYP56 (Dit2p) in Candida albicans: characterization and investigation of its role in growth and antifungal drug susceptibility.

Authors:  N R Melo; G P Moran; A G S Warrilow; E Dudley; S N Smith; D J Sullivan; D C Lamb; D E Kelly; D C Coleman; S L Kelly
Journal:  Antimicrob Agents Chemother       Date:  2008-07-28       Impact factor: 5.191

6.  Quantification of the influence of extracellular laccase and intracellular reactions on the isomer-specific biotransformation of the xenoestrogen technical nonylphenol by the aquatic hyphomycete Clavariopsis aquatica.

Authors:  Claudia Martin; Philippe F X Corvini; Ralph Vinken; Charles Junghanns; Gudrun Krauss; Dietmar Schlosser
Journal:  Appl Environ Microbiol       Date:  2009-05-08       Impact factor: 4.792

7.  Arv1 regulates PM and ER membrane structure and homeostasis but is dispensable for intracellular sterol transport.

Authors:  Alexander G Georgiev; Jesper Johansen; Vidhya D Ramanathan; Yves Y Sere; Christopher T Beh; Anant K Menon
Journal:  Traffic       Date:  2013-06-06       Impact factor: 6.215

8.  NADPH cytochrome P-450 oxidoreductase and susceptibility to ketoconazole.

Authors:  K Venkateswarlu; D E Kelly; N J Manning; S L Kelly
Journal:  Antimicrob Agents Chemother       Date:  1998-07       Impact factor: 5.191

9.  Unravelling evolutionary strategies of yeast for improving galactose utilization through integrated systems level analysis.

Authors:  Kuk-Ki Hong; Wanwipa Vongsangnak; Goutham N Vemuri; Jens Nielsen
Journal:  Proc Natl Acad Sci U S A       Date:  2011-06-29       Impact factor: 11.205

10.  Purification, reconstitution, and inhibition of cytochrome P-450 sterol delta22-desaturase from the pathogenic fungus Candida glabrata.

Authors:  D C Lamb; S Maspahy; D E Kelly; N J Manning; A Geber; J E Bennett; S L Kelly
Journal:  Antimicrob Agents Chemother       Date:  1999-07       Impact factor: 5.191
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