Molecular characterization of human monoclonal antibodies specific for several HIV proteins: analysis of the VH3 family expression.

We have analyzed the heavy-chain variable (VH) region genes expressed by a panel of human monoclonal antibodies derived from an immunized volunteer, an AIDS patient and seropositive asymptomatic donors, and specific for HIV-1 env, pol and gag gene products. The third complementarity-determining regions show a high complexity with unconventional gene recombination events. Most of the VH genes utilized are also frequently encountered in other immune responses. Their sequences are, in general, typical of an antigen-driven immune response. Molecular mechanisms that generate high-affinity antibodies are then effective during HIV infection. Remarkably, VH3 family, which dominates the human antibody repertoire, is barely encountered among anti-HIV antibodies.