Literature DB >> 8534121

Detection of viable Cryptosporidium parvum oocysts by PCR.

C Wagner-Wiening1, P Kimmig.   

Abstract

PCR was used to detect and specifically identify a gene fragment from Cryptosporidium parvum. An 873-bp region of a 2,359-bp DNA fragment encoding a repetitive oocyst protein of C. parvum was shown to be specifically amplified in C. parvum. An excystation protocol before DNA extraction allowed the differentiation between live and dead Cryptosporidium parvum oocysts.

Entities:  

Mesh：

Year:  1995        PMID： 8534121      PMCID： PMC167765          DOI： 10.1128/aem.61.12.4514-4516.1995

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  10 in total

1.  A probable waterborne outbreak of cryptosporidiosis in the Sheffield area.

Authors:  B A Rush; P A Chapman; R W Ineson
Journal:  J Med Microbiol       Date:  1990-08       Impact factor: 2.472

2.  The safety of our drinking water: reason for concern but not alarm.

Authors:  B H Olson
Journal:  N Engl J Med       Date:  1989-05-25       Impact factor: 91.245

3.  Large community outbreak of cryptosporidiosis due to contamination of a filtered public water supply.

Authors:  E B Hayes; T D Matte; T R O'Brien; T W McKinley; G S Logsdon; J B Rose; B L Ungar; D M Word; P F Pinsky; M L Cummings
Journal:  N Engl J Med       Date:  1989-05-25       Impact factor: 91.245

4.  Isolation of Cryptosporidium oocysts and sporozoites using discontinuous sucrose and isopycnic Percoll gradients.

Authors:  M J Arrowood; C R Sterling
Journal:  J Parasitol       Date:  1987-04       Impact factor: 1.276

5.  Separation of Cryptosporidium species oocysts from feces by using a percoll discontinuous density gradient.

Authors:  E Waldman; S Tzipori; J R Forsyth
Journal:  J Clin Microbiol       Date:  1986-01       Impact factor: 5.948

6.  The effects of reducing conditions, medium, pH, temperature, and time on in vitro excystation of Cryptosporidium.

Authors:  R Fayer; R G Leek
Journal:  J Protozool       Date:  1984-11

7.  An outbreak of waterborne cryptosporidiosis caused by post-treatment contamination.

Authors:  H V Smith; W J Patterson; R Hardie; L A Greene; C Benton; W Tulloch; R A Gilmour; R W Girdwood; J C Sharp; G I Forbes
Journal:  Epidemiol Infect       Date:  1989-12       Impact factor: 2.451

8.  A 2359-base pair DNA fragment from Cryptosporidium parvum encoding a repetitive oocyst protein.

Authors:  N C Lally; G D Baird; S J McQuay; F Wright; J J Oliver
Journal:  Mol Biochem Parasitol       Date:  1992-11       Impact factor: 1.759

9.  [Cryptosporidium parvum and Giardia lamblia--incidence in surface and drinking water--significance and detection].

Authors:  C Wagner; P Kimmig
Journal:  Gesundheitswesen       Date:  1992-11

10.  Identification of Cryptosporidium oocysts in river water.

Authors:  J E Ongerth; H H Stibbs
Journal:  Appl Environ Microbiol       Date:  1987-04       Impact factor: 4.792
  10 in total
  13 in total

1.  Immunomagnetic capture PCR to detect viable Cryptosporidium parvum oocysts from environmental samples.

Authors:  M Q Deng; D O Cliver; T W Mariam
Journal:  Appl Environ Microbiol       Date:  1997-08       Impact factor: 4.792

2.  Putative cis-regulatory elements associated with heat shock genes activated during excystation of Cryptosporidium parvum.

Authors:  Benjamin Cohn; Patricio Manque; Ana M Lara; Myrna Serrano; Nihar Sheth; Gregory Buck
Journal:  PLoS One       Date:  2010-03-04       Impact factor: 3.240

3.  Development of a novel, rapid integrated Cryptosporidium parvum detection assay.

Authors:  D Kozwich; K A Johansen; K Landau; C A Roehl; S Woronoff; P A Roehl
Journal:  Appl Environ Microbiol       Date:  2000-07       Impact factor: 4.792

4.  Failure to differentiate Cryptosporidium parvum from C. meleagridis based on PCR amplification of eight DNA sequences.

Authors:  D Champliaud; P Gobet; M Naciri; O Vagner; J Lopez; J C Buisson; I Varga; G Harly; R Mancassola; A Bonnin
Journal:  Appl Environ Microbiol       Date:  1998-04       Impact factor: 4.792

5.  Sensitive and rapid detection of viable Giardia cysts and Cryptosporidium parvum oocysts in large-volume water samples with wound fiberglass cartridge filters and reverse transcription-PCR.

Authors:  C Kaucner; T Stinear
Journal:  Appl Environ Microbiol       Date:  1998-05       Impact factor: 4.792

6.  An assay combining cell culture with reverse transcriptase PCR to detect and determine the infectivity of waterborne Cryptosporidium parvum.

Authors:  P A Rochelle; D M Ferguson; T J Handojo; R De Leon; M H Stewart; R L Wolfe
Journal:  Appl Environ Microbiol       Date:  1997-05       Impact factor: 4.792

7.  Detection of Cryptosporidium parvum DNA in human feces by nested PCR.

Authors:  A B Balatbat; G W Jordan; Y J Tang; J Silva
Journal:  J Clin Microbiol       Date:  1996-07       Impact factor: 5.948

8.  Detection of infectious Cryptosporidium parvum oocysts in surface and filter backwash water samples by immunomagnetic separation and integrated cell culture-PCR.

Authors:  G D Di Giovanni; F H Hashemi; N J Shaw; F A Abrams; M W LeChevallier; M Abbaszadegan
Journal:  Appl Environ Microbiol       Date:  1999-08       Impact factor: 4.792

9.  beta-tubulin mRNA as a marker of Cryptosporidium parvum oocyst viability.

Authors:  G Widmer; E A Orbacz; S Tzipori
Journal:  Appl Environ Microbiol       Date:  1999-04       Impact factor: 4.792

10.  Quantitative-PCR assessment of Cryptosporidium parvum cell culture infection.

Authors:  George D Di Giovanni; Mark W LeChevallier
Journal:  Appl Environ Microbiol       Date:  2005-03       Impact factor: 4.792
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