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Literature DB >> 8531927

The size heterogeneity of human lysyl oxidase mRNA is due to alternate polyadenylation site and not alternate exon usage.

C D Boyd¹, T J Mariani, Y Kim, K Csiszar.

Abstract

We have isolated the entire gene coding for human lysyl oxidase. Coding and untranslated domains of human lysyl oxidase mRNA were found in 7 exons, distributed throughout approximately 14 kb of human genomic DNA. The appearance of exon sequences in lysyl oxidase mRNA in several human tissues was determined using a reverse transcriptase - PCR assay. In contrast to a previous report, this analysis has unambiguously shown that the size heterogeneity of lysyl oxidase mRNA was not due to alternate usage of any of the exons of the lysyl oxidase gene. Moreover, DNA sequence analysis of the entire 3.8 kb 3'-untranslated region (UTR) within exon 7 revealed multiple poly-adenylation sites which were shown to be differentially expressed in human skin fibroblasts. This differential usage of polyadenylation sites within the 3'-UTR explains the appearance of multiple lysyl oxidase mRNAs of different sizes.

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Year: 1995 PMID： 8531927 DOI： 10.1007/bf00986499

Source DB: PubMed Journal: Mol Biol Rep ISSN： 0301-4851 Impact factor: 2.316

23 in total

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Authors: P C Trackman; D Bedell-Hogan; J Tang; H M Kagan
Journal: J Biol Chem Date: 1992-04-25 Impact factor: 5.157

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Journal: Cell Date: 1987-01-16 Impact factor: 41.582

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Authors: H Kuivaniemi
Journal: Biochem J Date: 1985-09-15 Impact factor: 3.857

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Authors: H M Kagan; P C Trackman
Journal: Am J Respir Cell Mol Biol Date: 1991-09 Impact factor: 6.914

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Authors: M A Williams; H M Kagan
Journal: Anal Biochem Date: 1985-09 Impact factor: 3.365

6. Structure of the mouse lysyl oxidase gene.

Authors: S Contente; K Csiszar; K Kenyon; R M Friedman
Journal: Genomics Date: 1993-05 Impact factor: 5.736

7. The complete derived amino acid sequence of human lysyl oxidase and assignment of the gene to chromosome 5 (extensive sequence homology with the murine ras recision gene).

Authors: T J Mariani; P C Trackman; H M Kagan; R L Eddy; T B Shows; C D Boyd; S B Deak
Journal: Matrix Date: 1992-06

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Authors: H Kuivaniemi; E R Savolainen; K I Kivirikko
Journal: J Biol Chem Date: 1984-06-10 Impact factor: 5.157

9. Comparison of lysyl oxidase from bovine lung and aorta.

Authors: A L Cronlund; H M Kagan
Journal: Connect Tissue Res Date: 1986 Impact factor: 3.417

10. Molecular cloning of human lysyl oxidase and assignment of the gene to chromosome 5q23.3-31.2.

Authors: E R Hämäläinen; T A Jones; D Sheer; K Taskinen; T Pihlajaniemi; K I Kivirikko
Journal: Genomics Date: 1991-11 Impact factor: 5.736

7 in total

Review 1. Variation in the lysyl oxidase (LOX) gene is associated with keratoconus in family-based and case-control studies.

Authors: Yelena Bykhovskaya; Xiaohui Li; Irina Epifantseva; Talin Haritunians; David Siscovick; Anthony Aldave; Loretta Szczotka-Flynn; Sudha K Iyengar; Kent D Taylor; Jerome I Rotter; Yaron S Rabinowitz
Journal: Invest Ophthalmol Vis Sci Date: 2012-06-28 Impact factor: 4.799

2. Functional analysis of the promoter and first intron of the human lysyl oxidase gene.

Authors: K Csiszar; I Entersz; P C Trackman; D Samid; C D Boyd
Journal: Mol Biol Rep Date: 1996 Impact factor: 2.316

3. Large-scale concatenation cDNA sequencing.

Authors: W Yu; B Andersson; K C Worley; D M Muzny; Y Ding; W Liu; J Y Ricafrente; M A Wentland; G Lennon; R A Gibbs
Journal: Genome Res Date: 1997-04 Impact factor: 9.043

4. Differential regulation by a peroxisome proliferator of the different multifunctional proteins in guinea pig: cDNA cloning of the guinea pig D-specific multifunctional protein 2.

Authors: F Caira; M C Clémencet; M Cherkaoui-Malki; M Dieuaide-Noubhani; C Pacot; P P Van Veldhoven; N Latruffe
Journal: Biochem J Date: 1998-03-15 Impact factor: 3.857