Literature DB >> 8529888

Color selection with a hygromycin-resistance-based Escherichia coli-mycobacterial shuttle vector.

N S Howard1, J E Gomez, C Ko, W R Bishai.   

Abstract

Hygromycin-resistance (HyR)-based Escherichia coli-mycobacterial shuttle plasmids have high efficiencies of transformation and a broad mycobacterial host range. We have introduced a lacZ alpha (encoding the alpha-polypeptide fragment of beta-galactosidase (beta Gal))-multiple cloning site cassette into a HyR-based shuttle vector to generate a plasmid with nine unique cloning sites and the added feature of beta Gal color selection in appropriate E. coli host strains.

Entities:  

Mesh：

Substances：

Year:  1995        PMID： 8529888     DOI： 10.1016/0378-1119(95)00597-x

Source DB:  PubMed          Journal:  Gene        ISSN： 0378-1119            Impact factor:   3.688


  33 in total

1.  Evaluation of a temperature-restricted, mucosal tuberculosis vaccine in guinea pigs.

Authors:  Tuhina Gupta; Monica LaGatta; Shelly Helms; Rebecca L Pavlicek; Simon O Owino; Kaori Sakamoto; Tamas Nagy; Stephen B Harvey; Mark Papania; Stephanie Ledden; Kevin T Schultz; Candace McCombs; Frederick D Quinn; Russell K Karls
Journal:  Tuberculosis (Edinb)       Date:  2018-10-19       Impact factor: 3.131

2.  Mycobacterium bovis BCG response regulator essential for hypoxic dormancy.

Authors:  Calvin Boon; Thomas Dick
Journal:  J Bacteriol       Date:  2002-12       Impact factor: 3.490

3.  AsnB is involved in natural resistance of Mycobacterium smegmatis to multiple drugs.

Authors:  Huiping Ren; Jun Liu
Journal:  Antimicrob Agents Chemother       Date:  2006-01       Impact factor: 5.191

4.  Cloning, expression, and site-directed mutagenesis of the propene monooxygenase genes from Mycobacterium sp. strain M156.

Authors:  Chan K Chan Kwo Chion; Sarah E Askew; David J Leak
Journal:  Appl Environ Microbiol       Date:  2005-04       Impact factor: 4.792

5.  Exposure to antibiotics induces expression of the Mycobacterium tuberculosis sigF gene: implications for chemotherapy against mycobacterial persistors.

Authors:  T M Michele; C Ko; W R Bishai
Journal:  Antimicrob Agents Chemother       Date:  1999-02       Impact factor: 5.191

6.  Different roles of DosS and DosT in the hypoxic adaptation of Mycobacteria.

Authors:  Min-Ju Kim; Kwang-Jin Park; In-Jeong Ko; Young Min Kim; Jeong-Il Oh
Journal:  J Bacteriol       Date:  2010-07-30       Impact factor: 3.490

7.  Attenuation of virulence in Mycobacterium tuberculosis expressing a constitutively active iron repressor.

Authors:  Y C Manabe; B J Saviola; L Sun; J R Murphy; W R Bishai
Journal:  Proc Natl Acad Sci U S A       Date:  1999-10-26       Impact factor: 11.205

8.  The conserved hypothetical protein Rv0574c is required for cell wall integrity, stress tolerance, and virulence of Mycobacterium tuberculosis.

Authors:  Rajni Garg; Deeksha Tripathi; Sashi Kant; Harish Chandra; Rakesh Bhatnagar; Nirupama Banerjee
Journal:  Infect Immun       Date:  2014-10-13       Impact factor: 3.441

9.  Development of a new generation of vectors for gene expression, gene replacement, and protein-protein interaction studies in mycobacteria.

Authors:  Amit Parikh; Devanand Kumar; Yogesh Chawla; Krishna Kurthkoti; Shazia Khan; Umesh Varshney; Vinay K Nandicoori
Journal:  Appl Environ Microbiol       Date:  2013-01-11       Impact factor: 4.792

10.  A rifampin-hypersensitive mutant reveals differences between strains of Mycobacterium smegmatis and presence of a novel transposon, IS1623.

Authors:  David C Alexander; Joses R W Jones; Jun Liu
Journal:  Antimicrob Agents Chemother       Date:  2003-10       Impact factor: 5.191
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.