Literature DB >> 8529885

Four new derivatives of the broad-host-range cloning vector pBBR1MCS, carrying different antibiotic-resistance cassettes.

M E Kovach1, P H Elzer, D S Hill, G T Robertson, M A Farris, R M Roop, K M Peterson.   

Abstract

Four new antibiotic-resistant derivatives of the broad-host-range (bhr) cloning vector pBBR1MCS have been constructed. These new plasmids have several advantages over many of the currently available bhr vectors in that: (i) they are relatively small (< 5.3 kb), (ii) they possess an extended multiple cloning site (MCS), (iii) they allow direct selection of recombinant plasmid molecules in Escherichia coli via disruption of the LacZ alpha peptide, (iv) they are mobilizable when the RK2 transfer functions are provided in trans and (v) they are compatible with IncP, IncQ and IncW group plasmids, as well as with ColE1- and P15a-based replicons.

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Year:  1995        PMID： 8529885     DOI： 10.1016/0378-1119(95)00584-1

Source DB:  PubMed          Journal:  Gene        ISSN： 0378-1119            Impact factor:   3.688


  1297 in total

1.  Identification of a plasmid-borne locus in Rhizobium etli KIM5s involved in lipopolysaccharide O-chain biosynthesis and nodulation of Phaseolus vulgaris.

Authors:  P Vinuesa; B L Reuhs; C Breton; D Werner
Journal:  J Bacteriol       Date:  1999-09       Impact factor: 3.490

2.  Signal-dependent DNA binding and functional domains of the quorum-sensing activator TraR as identified by repressor activity.

Authors:  Z Q Luo; S K Farrand
Journal:  Proc Natl Acad Sci U S A       Date:  1999-08-03       Impact factor: 11.205

3.  Role of phosphoglucomutase of Bordetella bronchiseptica in lipopolysaccharide biosynthesis and virulence.

Authors:  N P West; H Jungnitz; J T Fitter; J D McArthur; C A Guzmán; M J Walker
Journal:  Infect Immun       Date:  2000-08       Impact factor: 3.441

4.  Complex regulation of the organic hydroperoxide resistance gene (ohr) from Xanthomonas involves OhrR, a novel organic peroxide-inducible negative regulator, and posttranscriptional modifications.

Authors:  R Sukchawalit; S Loprasert; S Atichartpongkul; S Mongkolsuk
Journal:  J Bacteriol       Date:  2001-08       Impact factor: 3.490

5.  A dual reporter screening system identifies the amino acid at position 82 in Flp site-specific recombinase as a determinant for target specificity.

Authors:  Yuri Voziyanov; A Francis Stewart; Makkuni Jayaram
Journal:  Nucleic Acids Res       Date:  2002-04-01       Impact factor: 16.971

6.  Conditional-replication, integration, excision, and retrieval plasmid-host systems for gene structure-function studies of bacteria.

Authors:  A Haldimann; B L Wanner
Journal:  J Bacteriol       Date:  2001-11       Impact factor: 3.490

7.  The PtlE protein of Bordetella pertussis has peptidoglycanase activity required for Ptl-mediated pertussis toxin secretion.

Authors:  Amy A Rambow-Larsen; Alison A Weiss
Journal:  J Bacteriol       Date:  2002-06       Impact factor: 3.490

8.  Genetic analysis of pathway specificity during posttranslational protein translocation across the Escherichia coli plasma membrane.

Authors:  Natascha Blaudeck; Peter Kreutzenbeck; Roland Freudl; Georg A Sprenger
Journal:  J Bacteriol       Date:  2003-05       Impact factor: 3.490

9.  Identification and functional characterization of Sphingomonas macrogolitabida strain TFA genes involved in the first two steps of the tetralin catabolic pathway.

Authors:  Emilia Moreno-Ruiz; María José Hernáez; Olga Martínez-Pérez; Eduardo Santero
Journal:  J Bacteriol       Date:  2003-03       Impact factor: 3.490

10.  A Hybrid Extracellular Electron Transfer Pathway Enhances the Survival of Vibrio natriegens.

Authors:  Bridget E Conley; Matthew T Weinstock; Daniel R Bond; Jeffrey A Gralnick
Journal:  Appl Environ Microbiol       Date:  2020-09-17       Impact factor: 4.792
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