Literature DB >> 8528091

Crystallization and preliminary X-ray crystallographic analysis of arylesterase from Vibrio mimicus.

F N Musayev1, Y L Lee, J F Shaw, Y C Liaw.   

Abstract

Single crystals of arylesterase (EC 3.1.1.2) from Vibrio mimicus have been obtained from ammonium sulfate as a precipitant at room temperature for 2 months. The present crystals diffract up to 2.2 A resolution and belong to monoclinic space group P2(1). The cell dimensions are a = 55.65(1) A, b = 53.46(1) A, c = 65.79(1) A, and beta = 106.54(1) degrees. There are two molecules of molecular weight 22 kDa in an asymmetric unit with a solvent content of 43%.

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Year:  1995        PMID: 8528091      PMCID: PMC2143213          DOI: 10.1002/pro.5560040928

Source DB:  PubMed          Journal:  Protein Sci        ISSN: 0961-8368            Impact factor:   6.725


  5 in total

1.  Structure of human pancreatic lipase.

Authors:  F K Winkler; A D'Arcy; W Hunziker
Journal:  Nature       Date:  1990-02-22       Impact factor: 49.962

2.  Solvent content of protein crystals.

Authors:  B W Matthews
Journal:  J Mol Biol       Date:  1968-04-28       Impact factor: 5.469

3.  Nucleotide sequence of a novel arylesterase gene from Vibro mimicus and characterization of the enzyme expressed in Escherichia coli.

Authors:  J F Shaw; R C Chang; K H Chuang; Y T Yen; Y J Wang; F G Wang
Journal:  Biochem J       Date:  1994-03-15       Impact factor: 3.857

4.  Ser-His-Glu triad forms the catalytic site of the lipase from Geotrichum candidum.

Authors:  J D Schrag; Y G Li; S Wu; M Cygler
Journal:  Nature       Date:  1991-06-27       Impact factor: 49.962

5.  Cloning and nucleotide sequence of an esterase gene from Pseudomonas fluorescens and expression of the gene in Escherichia coli.

Authors:  K D Choi; G H Jeohn; J S Rhee; O J Yoo
Journal:  Agric Biol Chem       Date:  1990-08
  5 in total

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