| Literature DB >> 8521746 |
C W Canal1, H M Maia, I S Vaz Júnior, J M Chies, N A Farias, A Masuda, J C Gonzales, L S Ozaki, H Dewes.
Abstract
The major components of protein extracts from the cattle tick Boophilus microplus eggs and larvae of various ages were characterized by molecular sieving chromatography, ion exchange chromatography and SDS-PAGE. The fractions analysed showed a changing chromatographic pattern development. A serum raised against the components of a fraction showing characteristics of vitellin strongly reacted in Western blots with the major peptides of extracts from eggs, larvae, gut and ovary. Comparison of patterns obtained by electrophoresis in non-denaturing PAGE, stained with Coomassie blue or with benzidine/hydrogen peroxide, revealed that the major proteins of these extracts are haemoproteins, possibly in different aggregation states or heterogeneous in composition.Entities:
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Year: 1995 PMID: 8521746 DOI: 10.1007/bf00052390
Source DB: PubMed Journal: Exp Appl Acarol ISSN: 0168-8162 Impact factor: 2.132