Literature DB >> 852130

The measurement of serum free insulin by steady-state gel filtration.

C M Asplin, D J Goldie, M Hartog.   

Abstract

A method for the separation of bound and free insulin in the serum of insulin treated diabetics by steady state gel filtration is described. The method meets the criteria which have been suggested for the validation of a method of separating bound from free ligand. The precision of the method is satisfactory, and the results compare well with those obtained by the methods of ethanol precipitation and polyethylene glycol precipitation.

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Year:  1977        PMID： 852130     DOI： 10.1016/0009-8981(77)90358-8

Source DB:  PubMed          Journal:  Clin Chim Acta        ISSN： 0009-8981            Impact factor:   3.786


  3 in total

1.  Serum free insulin concentrations during the treatment of diabetic coma and precoma with low dose intramuscular insulin.

Authors:  C M Asplin; M Hartog
Journal:  Diabetologia       Date:  1977-09       Impact factor: 10.122

2.  Change of insulin dosage, circulating free and bound insulin and insulin antibodies on transferring diabetics from conventional to highly purified porcine insulin.

Authors:  C M Asplin; M Hartog; D J Goldie
Journal:  Diabetologia       Date:  1978-02       Impact factor: 10.122

3.  Measurement of free insulin concentrations: the influence of the timing of extraction of insulin antibodies.

Authors:  I Hanning; P D Home; K G Alberti
Journal:  Diabetologia       Date:  1985-11       Impact factor: 10.122
  3 in total

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