Literature DB >> 8521036

Precise large deletions by the PCR-based overlap extension method.

S D Senanayake1, D A Brian.   

Abstract

The authors describe an efficient method for generating large deletions (> 200 nts) of precise length using the PCR-based method of gene splicing by overlap extension (1). This method is technically simple and less time consuming than conventional loop-out mutagenesis techniques requiring preparation of a single-stranded DNA template.

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Year:  1995        PMID： 8521036      PMCID： PMC7090436          DOI： 10.1007/bf02907467

Source DB:  PubMed          Journal:  Mol Biotechnol        ISSN： 1073-6085            Impact factor:   2.695


  3 in total

1.  Gene splicing by overlap extension: tailor-made genes using the polymerase chain reaction.

Authors:  R M Horton; Z L Cai; S N Ho; L R Pease
Journal:  Biotechniques       Date:  1990-05       Impact factor: 1.993

2.  Rapid and efficient site-specific mutagenesis without phenotypic selection.

Authors:  T A Kunkel; J D Roberts; R A Zakour
Journal:  Methods Enzymol       Date:  1987       Impact factor: 1.600

3.  Oligonucleotide-directed mutagenesis: a simple method using two oligonucleotide primers and a single-stranded DNA template.

Authors:  M J Zoller; M Smith
Journal:  Methods Enzymol       Date:  1987       Impact factor: 1.600
  3 in total
  41 in total

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Authors:  S D Senanayake; D A Brian
Journal:  J Virol       Date:  1999-10       Impact factor: 5.103

2.  A phylogenetically conserved hairpin-type 3' untranslated region pseudoknot functions in coronavirus RNA replication.

Authors:  G D Williams; R Y Chang; D A Brian
Journal:  J Virol       Date:  1999-10       Impact factor: 5.103

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Authors:  Anna D Tischler; Sang Ho Lee; Andrew Camilli
Journal:  J Bacteriol       Date:  2002-08       Impact factor: 3.490

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Authors:  Alejandro F Alice; Jorge H Crosa
Journal:  J Bacteriol       Date:  2012-02-03       Impact factor: 3.490

5.  Reactivation of the vanchrobactin siderophore system of Vibrio anguillarum by removal of a chromosomal insertion sequence originated in plasmid pJM1 encoding the anguibactin siderophore system.

Authors:  Hiroaki Naka; Claudia S López; Jorge H Crosa
Journal:  Environ Microbiol       Date:  2007-11-13       Impact factor: 5.491

6.  A rapid and efficient method for site-directed mutagenesis using one-step overlap extension PCR.

Authors:  A Urban; S Neukirchen; K E Jaeger
Journal:  Nucleic Acids Res       Date:  1997-06-01       Impact factor: 16.971

7.  Cellular protein HAX1 interacts with the influenza A virus PA polymerase subunit and impedes its nuclear translocation.

Authors:  Wei-Bin Hsu; Jia-Ling Shih; Jie-Ru Shih; Jia-Ling Du; Shu-Chun Teng; Li-Min Huang; Won-Bo Wang
Journal:  J Virol       Date:  2012-10-10       Impact factor: 5.103

8.  An RNA stem-loop within the bovine coronavirus nsp1 coding region is a cis-acting element in defective interfering RNA replication.

Authors:  Cary G Brown; Kimberley S Nixon; Savithra D Senanayake; David A Brian
Journal:  J Virol       Date:  2007-05-02       Impact factor: 5.103

9.  Mycobacterium tuberculosis Requires Regulation of ESX-5 Secretion for Virulence in Irgm1-Deficient Mice.

Authors:  Sarah R Elliott; Dylan W White; Anna D Tischler
Journal:  Infect Immun       Date:  2019-01-24       Impact factor: 3.441

10.  An EGFR gene of the Pacific oyster Crassostrea gigas functions in wound healing and promotes cell proliferation.

Authors:  Lingling Sun; Pin Huan; Hongxia Wang; Fengsong Liu; Baozhong Liu
Journal:  Mol Biol Rep       Date:  2014-01-28       Impact factor: 2.316
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