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Literature DB >> 8517708

Use of an in vivo system to determine the G418 resistance phenotype of bloodstream-form Trypanosoma brucei brucei transfectants.

N B Murphy¹, A M Muthiani, A S Peregrine.

Abstract

We determined the level of susceptibility of Trypanosoma brucei brucei to the aminoglycoside G418 in vivo and demonstrated that it is possible to select for G418-resistant transfected T. brucei brucei bloodstream parasites in a mouse host by inoculating the drug intraperitoneally at doses between 40 and 80 mg/kg of body weight daily for 3 days. The ability to select for transfectants in vivo offers new possibilities for studies on genetic recombination in these parasites.

Entities: Chemical Species

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Year: 1993 PMID： 8517708 PMCID： PMC187924 DOI： 10.1128/AAC.37.5.1167

Source DB: PubMed Journal: Antimicrob Agents Chemother ISSN： 0066-4804 Impact factor: 5.191

18 in total

1. The cultivation of Trypanosoma congolense in vitro.

Authors: E J TOBIE
Journal: J Parasitol Date: 1958-04 Impact factor: 1.276

2. High-efficiency clonal growth of bloodstream- and insect-form Trypanosoma brucei on agarose plates.

Authors: V B Carruthers; G A Cross
Journal: Proc Natl Acad Sci U S A Date: 1992-09-15 Impact factor: 11.205

3. Stable integrative transformation of Trypanosoma brucei that occurs exclusively by homologous recombination.

Authors: J Eid; B Sollner-Webb
Journal: Proc Natl Acad Sci U S A Date: 1991-03-15 Impact factor: 11.205

4. Homologous recombination and stable transfection in the parasitic protozoan Trypanosoma brucei.

Authors: M G Lee; L H Van der Ploeg
Journal: Science Date: 1990-12-14 Impact factor: 47.728

5. Continuous cultivation of Trypanosoma brucei blood stream forms in a medium containing a low concentration of serum protein without feeder cell layers.

Authors: H Hirumi; K Hirumi
Journal: J Parasitol Date: 1989-12 Impact factor: 1.276

6. Three small RNAs within the 10 kb trypanosome rRNA transcription unit are analogous to domain VII of other eukaryotic 28S rRNAs.

Authors: T C White; G Rudenko; P Borst
Journal: Nucleic Acids Res Date: 1986-12-09 Impact factor: 16.971

7. The epidemiology of Trypanosoma rhodesiense sleeping sickness in Alego location, Central Nyanza, Kenya. I. Evidence that cattle may act as reservoir hosts of trypanosomes infective to man.

Authors: R J Onyango; K Van Hoeve; P De Raadt
Journal: Trans R Soc Trop Med Hyg Date: 1966 Impact factor: 2.184

8. Disulfide bond involvement in the maintenance of the cryptic nature of the cross-reacting determinant of metacyclic forms of Trypanosoma congolense.

Authors: W R Fish; C W Muriuki; A M Muthiani; D J Grab; J D Lonsdale-Eccles
Journal: Biochemistry Date: 1989-06-27 Impact factor: 3.162

Use of an in vivo system to determine the G418 resistance phenotype of bloodstream-form Trypanosoma brucei brucei transfectants.

1. The cultivation of Trypanosoma congolense in vitro.

2. High-efficiency clonal growth of bloodstream- and insect-form Trypanosoma brucei on agarose plates.

3. Stable integrative transformation of Trypanosoma brucei that occurs exclusively by homologous recombination.

4. Homologous recombination and stable transfection in the parasitic protozoan Trypanosoma brucei.

5. Continuous cultivation of Trypanosoma brucei blood stream forms in a medium containing a low concentration of serum protein without feeder cell layers.

6. Three small RNAs within the 10 kb trypanosome rRNA transcription unit are analogous to domain VII of other eukaryotic 28S rRNAs.

7. The epidemiology of Trypanosoma rhodesiense sleeping sickness in Alego location, Central Nyanza, Kenya. I. Evidence that cattle may act as reservoir hosts of trypanosomes infective to man.

8. Disulfide bond involvement in the maintenance of the cryptic nature of the cross-reacting determinant of metacyclic forms of Trypanosoma congolense.

9. Targeted insertion of the neomycin phosphotransferase gene into the tubulin gene cluster of Trypanosoma brucei.

10. The hygromycin B-resistance-encoding gene as a selectable marker for stable transformation of Trypanosoma brucei.

1. DNA rearrangements associated with multiple consecutive directed antigenic switches in Trypanosoma brucei.

2. Using T. brucei as a biological epitope-display platform to elicit specific antibody responses.

3. Use of in vivo and in vitro systems to select Leishmania amazonensis expressing green fluorescent protein.