Literature DB >> 8514199

High-level production of biologically active human cytosolic phospholipase A2 in baculovirus-infected insect cells.

B Y Amegadzie1, D Jiampetti, R J Craig, E Appelbaum, A R Shatzman, R J Mayer, A G DiLella.   

Abstract

Infection of Spodoptera frugiperda insect cells with a recombinant baculovirus expressing human cytosolic phospholipase A2 (cPLA2) resulted in the production of biologically active protein. The level of recombinant human cPLA2 production in infected insect cells was at least 50-fold higher than that observed in human monoblast U937 cells.

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Year:  1993        PMID: 8514199     DOI: 10.1016/0378-1119(93)90580-v

Source DB:  PubMed          Journal:  Gene        ISSN: 0378-1119            Impact factor:   3.688


  1 in total

1.  Prostaglandin E2 requirement for transforming growth factor beta 1 inhibition of elicited macrophage 14 kDa phospholipase A2 release.

Authors:  M McCord; B Bolognese; L A Marshall
Journal:  Br J Pharmacol       Date:  1995-11       Impact factor: 8.739

  1 in total

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