In vitro stimulation of human fetal lymphocytes by mitogens and interleukins.

Human lymphocytes derived from fetal spleen and liver were studied for their capacity to respond to mitogens and interleukins using different in vitro models (cell volume increase, [3H]thymidine incorporation, Ig secretion). Although the number of mature B and T cells in the fetal liver preparations remained nearly constant [Settmacher et al. (1991) Immunobiol. 182, 256], only lymphocytes obtained from fetal organisms before the 25th week of gestation could respond to some of the polyclonal stimulators (PWM, anti-CD3 + IL-2, SAC + IL-2, SAC + IL-4) tested, whereas cells obtained after that period failed. In the fetal spleen, however, with increasing percentages of mature B and T cells during fetal development, a growing ability to respond to mitogens was registered, which, however, did not achieve the values found for the adult spleen material.