Literature DB >> 8507829

Identification of a RAPD marker associated with somatic embryogenesis in alfalfa.

K Yu1, K P Pauls.   

Abstract

The current study was conducted to identify random amplified polymorphic DNA (RAPD) markers linked to genes controlling somatic embryogenesis in alfalfa. Segregation analyses of the somatic embryogenesis trait and the RAPD markers in an F1 population of 83 plants, derived from a cross between embryogenic A70-34 and non-embryogenic Arrow36 alfalfa plants, identified a polymorphic band that is associated with somatic embryogenesis. Based on the assumptions that somatic embryogenesis in alfalfa is controlled by two dominant genes with complementary effects and that the genotypes of A70-34 and Arrow36 are AAaaBbbb and aaaabbbb, respectively, the segregation data for the marker and the somatic embryogenesis trait in the F1s indicate that the marker is linked to the A locus. The maximum recombination fraction estimated for the linkage between the marker and the gene is 36.3%.

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Year:  1993        PMID： 8507829     DOI： 10.1007/BF00014934

Source DB:  PubMed          Journal:  Plant Mol Biol        ISSN： 0167-4412            Impact factor:   4.076


  7 in total

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Journal:  Theor Appl Genet       Date:  1992-08       Impact factor: 5.699

5.  DNA polymorphisms amplified by arbitrary primers are useful as genetic markers.

Authors:  J G Williams; A R Kubelik; K J Livak; J A Rafalski; S V Tingey
Journal:  Nucleic Acids Res       Date:  1990-11-25       Impact factor: 16.971

6.  Resolution of quantitative traits into Mendelian factors by using a complete linkage map of restriction fragment length polymorphisms.

Authors:  A H Paterson; E S Lander; J D Hewitt; S Peterson; S E Lincoln; S D Tanksley
Journal:  Nature       Date:  1988-10-20       Impact factor: 49.962

7.  Rapid identification of markers linked to a Pseudomonas resistance gene in tomato by using random primers and near-isogenic lines.

Authors:  G B Martin; J G Williams; S D Tanksley
Journal:  Proc Natl Acad Sci U S A       Date:  1991-03-15       Impact factor: 11.205
  7 in total
  9 in total

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Authors:  P W Taylor; J R Geijskes; H L Ko; T A Fraser; R J Henry; R G Birch
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5.  Genetic mapping of QTLs controlling vegetative propagation in Eucalyptus grandis and E. urophylla using a pseudo-testcross strategy and RAPD markers.

Authors:  D Grattapaglia; F L Bertolucci; R R Sederoff
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6.  New potential markers of in vitro tomato morphogenesis identified by mRNA differential display.

Authors:  A Torelli; E Soragni; A Bolchi; S Petrucco; S Ottonello; C Branca
Journal:  Plant Mol Biol       Date:  1996-12       Impact factor: 4.076

7.  Characterization of somatic embryogenesis-related cDNAs from alfalfa (Medicago sativa L.).

Authors:  R W Giroux; K P Pauls
Journal:  Plant Mol Biol       Date:  1997-02       Impact factor: 4.076

8.  Genetic improvement of the somatic embryogenesis and regeneration in soybean and transformation of the improved breeding lines.

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9.  Mapping QTLs that control the performance of rice tissue culture and evaluation of derived near-isogenic lines.

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  9 in total

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