Literature DB >> 8506941

Two types of putative preneoplastic lesions identified by hexosaminidase activity in whole-mounts of colons from F344 rats treated with carcinogen.

T P Pretlow1, M A O'Riordan, K M Spancake, T G Pretlow.   

Abstract

Previous studies identified as putative preneoplastic lesions 1) enzyme-altered foci in sections of methacrylate-embedded colon and 2) aberrant crypts in methylene blue-stained unembedded (whole-mount) colon and established that aberrant crypts embedded in methacrylate had enzyme alterations. We have now studied histochemically demonstrable hexosaminidase activity in unembedded or whole-mount preparations of colons from carcinogen-treated rats. These preparations have revealed two populations of crypts that are enzyme-altered: those that are morphologically altered or aberrant and those that are morphologically normal. Both populations can be quantified rigorously in less than an hour with whole-mount preparations reacted for hexosaminidase. The demonstration of phenotypic characteristics with histochemical techniques in whole-mount preparations should have wide applicability to functional studies in many normal and diseased tissues.

Entities:  

Mesh：

Substances：

Year:  1993        PMID： 8506941      PMCID： PMC1887005     

Source DB:  PubMed          Journal:  Am J Pathol        ISSN： 0002-9440            Impact factor:   4.307


  37 in total

1.  The natural history of atherosclerosis: the early aortic lesions as seen in New Orleans in the middle of the of the 20th century.

Authors:  R L HOLMAN; H C McGILL; J P STRONG; J C GEER
Journal:  Am J Pathol       Date:  1958 Mar-Apr       Impact factor: 4.307

2.  Examination of enzyme-altered foci with gamma-glutamyl transpeptidase, aldehyde dehydrogenase, glucose-6-phosphate dehydrogenase, and other markers in methacrylate-embedded liver.

Authors:  T P Pretlow; R W Grane; P L Goehring; A S Lapinsky; T G Pretlow
Journal:  Lab Invest       Date:  1987-01       Impact factor: 5.662

3.  A clinicopathological study of minute polypoid lesions of the colon based on magnifying fiber-colonoscopy and dissecting microscopy.

Authors:  M Nishizawa; T Okada; F Sato; A Kariya; S Mayama; K Nakamura
Journal:  Endoscopy       Date:  1980-05       Impact factor: 10.093

4.  Small "flat adenoma" of the large bowel with special reference to its clinicopathologic features.

Authors:  T Muto; J Kamiya; T Sawada; F Konishi; K Sugihara; Y Kubota; M Adachi; S Agawa; Y Saito; Y Morioka
Journal:  Dis Colon Rectum       Date:  1985-11       Impact factor: 4.585

5.  Regional assignment of genes for human esterase D and retinoblastoma to chromosome band 13q14.

Authors:  R S Sparkes; M C Sparkes; M G Wilson; J W Towner; W Benedict; A L Murphree; J J Yunis
Journal:  Science       Date:  1980-05-30       Impact factor: 47.728

6.  Classification of aberrant crypt foci and microadenomas in human colon.

Authors:  L Roncucci; A Medline; W R Bruce
Journal:  Cancer Epidemiol Biomarkers Prev       Date:  1991 Nov-Dec       Impact factor: 4.254

7.  Nuclear DNA content in dimethylhydrazine-induced colonic carcinoma and mucosal dysplasia in rats.

Authors:  O Kimura; N Kaibara; Y Miyano; T Okamoto; H Tamura; E Yurugi; S Koga
Journal:  Cancer       Date:  1984-05-01       Impact factor: 6.860

8.  Gardner syndrome in a man with an interstitial deletion of 5q.

Authors:  L Herrera; S Kakati; L Gibas; E Pietrzak; A A Sandberg
Journal:  Am J Med Genet       Date:  1986-11

9.  Gene for hereditary retinoblastoma assigned to human chromosome 13 by linkage to esterase D.

Authors:  R S Sparkes; A L Murphree; R W Lingua; M C Sparkes; L L Field; S J Funderburk; W F Benedict
Journal:  Science       Date:  1983-02-25       Impact factor: 47.728

10.  Tay-Sachs' and Sandhoff's diseases: the assignment of genes for hexosaminidase A and B to individual human chromosomes.

Authors:  F Gilbert; R Kucherlapati; R P Creagan; M J Murnane; G J Darlington; F H Ruddle
Journal:  Proc Natl Acad Sci U S A       Date:  1975-01       Impact factor: 11.205
View more
  5 in total

Review 1.  Development and progression of colorectal neoplasia.

Authors:  Upender Manne; Chandrakumar Shanmugam; Venkat R Katkoori; Harvey L Bumpers; William E Grizzle
Journal:  Cancer Biomark       Date:  2010       Impact factor: 4.388

2.  Characterization of dysplastic aberrant crypt foci in the rat colon induced by 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine.

Authors:  Masako Ochiai; Mitsunori Ushigome; Kyoko Fujiwara; Tsuneyuki Ubagai; Toshihiko Kawamori; Takashi Sugimura; Minako Nagao; Hitoshi Nakagama
Journal:  Am J Pathol       Date:  2003-10       Impact factor: 4.307

Review 3.  Mouse models for the study of colon carcinogenesis.

Authors:  Daniel W Rosenberg; Charles Giardina; Takuji Tanaka
Journal:  Carcinogenesis       Date:  2008-11-26       Impact factor: 4.944

Review 4.  Morphological and molecular alterations in 1,2 dimethylhydrazine and azoxymethane induced colon carcinogenesis in rats.

Authors:  Martina Perše; Anton Cerar
Journal:  J Biomed Biotechnol       Date:  2010-12-28

5.  Hexosaminidase-altered aberrant crypts, carrying decreased hexosaminidase alpha and beta subunit mRNAs, in colon of 1,2-dimethylhydrazine-treated rats.

Authors:  T Tsukamoto; H Fukami; S Yamanaka; A Yamaguchi; H Nakanishi; H Sakai; I Aoki; M Tatematsu
Journal:  Jpn J Cancer Res       Date:  2001-02
  5 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.