Literature DB >> 8505698

Enzyme immunoassay for respiratory syncytial virus: rapid detection in nasopharyngeal secretions and evaluation of isolates representing different RSV subgroups.

M M Siqueira1, J P Nascimento, S A Portes, W Schuy.   

Abstract

The presence of respiratory syncytial virus (RSV) was investigated by immunofluorescent antibody (IFA) technique and by an enzyme immunoassay (EIA) in 169 samples of nasopharyngeal secretions of infants and children with acute respiratory infections. Of 31 samples positive by EIA, 25 were positive by IFA. In 24 samples from a retrospective study, RSV positive by IFA and/or tissue culture isolation (TCI), 22 were also positive by EIA. The EIA was also evaluated with 111 RSV isolates in Hep2 cell cultures representing different RSV subgroups. All were positive by EIA.

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Year:  1993        PMID： 8505698      PMCID： PMC7166710          DOI： 10.1002/jcla.1860070212

Source DB:  PubMed          Journal:  J Clin Lab Anal        ISSN： 0887-8013            Impact factor:   2.352


  19 in total

1.  Two distinct subtypes of human respiratory syncytial virus.

Authors:  M A Mufson; C Orvell; B Rafnar; E Norrby
Journal:  J Gen Virol       Date:  1985-10       Impact factor: 3.891

2.  Antigenic characterization of respiratory syncytial virus strains with monoclonal antibodies.

Authors:  L J Anderson; J C Hierholzer; C Tsou; R M Hendry; B F Fernie; Y Stone; K McIntosh
Journal:  J Infect Dis       Date:  1985-04       Impact factor: 5.226

Review 3.  Rapid laboratory techniques for the diagnosis of viral infections.

Authors: 
Journal:  World Health Organ Tech Rep Ser       Date:  1981

4.  Detection of respiratory syncytial virus in nasopharyngeal secretions by enzyme-linked immunosorbent assay, indirect immunofluorescence, and virus isolation: a comparative study.

Authors:  T Popow-Kraupp; G Kern; C Binder; W Tuma; M Kundi; C Kunz
Journal:  J Med Virol       Date:  1986-06       Impact factor: 2.327

5.  Detection of respiratory syncytial, parainfluenza type 2, and adenovirus antigens by radioimmunoassay and enzyme immunoassay on nasopharyngeal specimens from children with acute respiratory disease.

Authors:  H K Sarkkinen; P E Halonen; P P Arstila; A A Salmi
Journal:  J Clin Microbiol       Date:  1981-02       Impact factor: 5.948

6.  Antigenic relatedness between glycoproteins of human respiratory syncytial virus subgroups A and B: evaluation of the contributions of F and G glycoproteins to immunity.

Authors:  P R Johnson; R A Olmsted; G A Prince; B R Murphy; D W Alling; E E Walsh; P L Collins
Journal:  J Virol       Date:  1987-10       Impact factor: 5.103

7.  Detection of respiratory syncytial virus in clinical specimens by viral culture, direct and indirect immunofluorescence, and enzyme immunoassay.

Authors:  J H Hughes; D R Mann; V V Hamparian
Journal:  J Clin Microbiol       Date:  1988-03       Impact factor: 5.948

8.  RS virus diagnosis: comparison of isolation, immunofluorescence and enzyme immunoassay.

Authors:  M M Siqueira; V Ferreira; J P Nascimento
Journal:  Mem Inst Oswaldo Cruz       Date:  1986 Apr-Jun       Impact factor: 2.743

9.  Comparison of washed nasopharyngeal cells and whole nasal secretions for detection of respiratory syncytial virus antigens by enzyme-linked immunosorbent assay.

Authors:  R M Hendry; L T Pierik; K McIntosh
Journal:  J Clin Microbiol       Date:  1986-02       Impact factor: 5.948

10.  Rapid viral diagnosis of acute respiratory infections: comparison of enzyme-linked immunosorbent assay and the immunofluorescence technique for detection of viral antigens in nasopharyngeal secretions.

Authors:  M Grandien; C A Pettersson; P S Gardner; A Linde; A Stanton
Journal:  J Clin Microbiol       Date:  1985-11       Impact factor: 5.948
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