Literature DB >> 8503954

Mutants in disulfide bond formation that disrupt flagellar assembly in Escherichia coli.

F E Dailey1, H C Berg.   

Abstract

We report the isolation and characterization of Escherichia coli mutants (dsbB) that fail to assemble functional flagella unless cystine is present. Flagellar basal bodies obtained from these mutants are missing the L and P rings. This defect in assembly appears to result from an inability to form a disulfide bond in the P-ring protein (FlgI). Cystine suppresses this defect in dsbB strains. We also show that dsbA strains [Bardwell, J. C. A., McGovern, K. & Beckwith, J. (1991) Cell 67, 581-589] fail to assemble P rings, apparently from a similar failure in disulfide bond formation. However, cystine does not completely suppress this defect in dsbA strains. Thus, disulfide bond formation in FlgI is essential for assembly. DsbA likely puts in that bond directly, whereas the DsbB product(s) play a role in oxidizing DsbA, so that it can be active.

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Year:  1993        PMID： 8503954      PMCID： PMC45807          DOI： 10.1073/pnas.90.3.1043

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  37 in total

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Authors:  Y Komeda; T Iino
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4.  Complementation analysis and deletion mapping of Escherichia coli mutants defective in chemotaxis.

Authors:  J S Parkinson
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Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

6.  A pathway for disulfide bond formation in vivo.

Authors:  J C Bardwell; J O Lee; G Jander; N Martin; D Belin; J Beckwith
Journal:  Proc Natl Acad Sci U S A       Date:  1993-02-01       Impact factor: 11.205

7.  Conformational changes associated with proteolytic processing of presecretory proteins allow glutathione-catalyzed formation of native disulfide bonds.

Authors:  G Scheele; R Jacoby
Journal:  J Biol Chem       Date:  1982-10-25       Impact factor: 5.157

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Authors:  S R Maloy; W D Nunn
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Authors:  M Garnak; H C Reeves
Journal:  J Biol Chem       Date:  1979-08-25       Impact factor: 5.157

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Authors:  Y Komeda; M Silverman; P Matsumura; M Simon
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  110 in total

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Authors:  Frank E Dailey; Robert M Macnab
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8.  DegP is involved in Cpx-mediated posttranscriptional regulation of the type III secretion apparatus in enteropathogenic Escherichia coli.

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Review 9.  The thioredoxin superfamily: redundancy, specificity, and gray-area genomics.

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Journal:  J Bacteriol       Date:  1999-03       Impact factor: 3.490

10.  Identification and characterization of the Escherichia coli gene dsbB, whose product is involved in the formation of disulfide bonds in vivo.

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Journal:  Proc Natl Acad Sci U S A       Date:  1993-08-01       Impact factor: 11.205
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