Literature DB >> 8503193

Localization and biochemical characterization of alfalfa mosaic virus replication complexes.

M De Graaff1, L Coscoy, E M Jaspars.   

Abstract

Replication complexes were isolated from alfalfa mosaic virus-infected tobacco protoplasts. Most of the RNA-synthesizing activity appears to colocalize with the intact chloroplasts upon sucrose-gradient centrifugation of cellular homogenates. Further analysis of these replication complexes showed that the enzyme is strongly associated with the outside of the chloroplasts, the endogenous template being well protected against ribonuclease action. RNA polymerase activity is sensitive to protease treatment of intact chloroplast fraction showing that an essential part of the enzyme complex is facing the in vitro medium, and probably the cytosol in vivo.

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Year:  1993        PMID： 8503193     DOI： 10.1006/viro.1993.1335

Source DB:  PubMed          Journal:  Virology        ISSN： 0042-6822            Impact factor:   3.616


  19 in total

1.  The tobacco mosaic virus RNA polymerase complex contains a plant protein related to the RNA-binding subunit of yeast eIF-3.

Authors:  T A Osman; K W Buck
Journal:  J Virol       Date:  1997-08       Impact factor: 5.103

2.  Complex dynamic development of poliovirus membranous replication complexes.

Authors:  George A Belov; Vinod Nair; Bryan T Hansen; Forrest H Hoyt; Elizabeth R Fischer; Ellie Ehrenfeld
Journal:  J Virol       Date:  2011-11-09       Impact factor: 5.103

3.  Immunodetection of the 33 K/92 K polymerase proteins in cymbidium ringspot virus-infected and in transgenic plant tissue extracts.

Authors:  R Lupo; L Rubino; M Russo
Journal:  Arch Virol       Date:  1994       Impact factor: 2.574

4.  Accumulation of alfalfa mosaic virus RNAs 1 and 2 requires the encoded proteins in cis.

Authors:  C M van Rossum; M L Garcia; J F Bol
Journal:  J Virol       Date:  1996-08       Impact factor: 5.103

5.  Synthesis of potato virus X RNAs by membrane-containing extracts.

Authors:  S V Doronin; C Hemenway
Journal:  J Virol       Date:  1996-07       Impact factor: 5.103

6.  Brome mosaic virus helicase- and polymerase-like proteins colocalize on the endoplasmic reticulum at sites of viral RNA synthesis.

Authors:  M A Restrepo-Hartwig; P Ahlquist
Journal:  J Virol       Date:  1996-12       Impact factor: 5.103

7.  TOM1, an Arabidopsis gene required for efficient multiplication of a tobamovirus, encodes a putative transmembrane protein.

Authors:  T Yamanaka; T Ohta; M Takahashi; T Meshi; R Schmidt; C Dean; S Naito; M Ishikawa
Journal:  Proc Natl Acad Sci U S A       Date:  2000-08-29       Impact factor: 11.205

8.  Open reading frame 1a-encoded subunits of the arterivirus replicase induce endoplasmic reticulum-derived double-membrane vesicles which carry the viral replication complex.

Authors:  K W Pedersen; Y van der Meer; N Roos; E J Snijder
Journal:  J Virol       Date:  1999-03       Impact factor: 5.103

9.  Alfalfa mosaic virus replicase proteins P1 and P2 interact and colocalize at the vacuolar membrane.

Authors:  M W Van Der Heijden; J E Carette; P J Reinhoud; A Haegi; J F Bol
Journal:  J Virol       Date:  2001-02       Impact factor: 5.103

10.  ORF1a-encoded replicase subunits are involved in the membrane association of the arterivirus replication complex.

Authors:  Y van der Meer; H van Tol; J K Locker; E J Snijder
Journal:  J Virol       Date:  1998-08       Impact factor: 5.103
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