Literature DB >> 8502550

Sequence selective double strand DNA cleavage by peptide nucleic acid (PNA) targeting using nuclease S1.

V Demidov1, M D Frank-Kamenetskii, M Egholm, O Buchardt, P E Nielsen.   

Abstract

A novel method for sequence specific double strand DNA cleavage using PNA (peptide nucleic acid) targeting is described. Nuclease S1 digestion of double stranded DNA gives rise to double strand cleavage at an occupied PNA strand displacement binding site, and under optimized conditions complete cleavage can be obtained. The efficiency of this cleavage is more than 10 fold enhanced when a tandem PNA site is targeted, and additionally enhanced if this site is in trans rather than in cis orientation. Thus in effect, the PNA targeting makes the single strand specific nuclease S1 behave like a pseudo restriction endonuclease.

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Year:  1993        PMID： 8502550      PMCID： PMC309471          DOI： 10.1093/nar/21.9.2103

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  11 in total

1.  Second structural motif for recognition of DNA by oligonucleotide-directed triple-helix formation.

Authors:  P A Beal; P B Dervan
Journal:  Science       Date:  1991-03-15       Impact factor: 47.728

2.  Site-specific cleavage of a yeast chromosome by oligonucleotide-directed triple-helix formation.

Authors:  S A Strobel; P B Dervan
Journal:  Science       Date:  1990-07-06       Impact factor: 47.728

3.  Sequence-specific recognition and cleavage of duplex DNA via triple-helix formation by oligonucleotides covalently linked to a phenanthroline-copper chelate.

Authors:  J C François; T Saison-Behmoaras; C Barbier; M Chassignol; N T Thuong; C Hélène
Journal:  Proc Natl Acad Sci U S A       Date:  1989-12       Impact factor: 11.205

4.  Cleaving yeast and Escherichia coli genomes at a single site.

Authors:  M Koob; W Szybalski
Journal:  Science       Date:  1990-10-12       Impact factor: 47.728

5.  Sequence-specific artificial photo-induced endonucleases based on triple helix-forming oligonucleotides.

Authors:  L Perrouault; U Asseline; C Rivalle; N T Thuong; E Bisagni; C Giovannangeli; T Le Doan; C Hélène
Journal:  Nature       Date:  1990-03-22       Impact factor: 49.962

6.  Single-site enzymatic cleavage of yeast genomic DNA mediated by triple helix formation.

Authors:  S A Strobel; P B Dervan
Journal:  Nature       Date:  1991-03-14       Impact factor: 49.962

7.  DNA unwinding upon strand-displacement binding of a thymine-substituted polyamide to double-stranded DNA.

Authors:  D Y Cherny; B P Belotserkovskii; M D Frank-Kamenetskii; M Egholm; O Buchardt; R H Berg; P E Nielsen
Journal:  Proc Natl Acad Sci U S A       Date:  1993-03-01       Impact factor: 11.205

8.  Site-specific inhibition of EcoRI restriction/modification enzymes by a DNA triple helix.

Authors:  J C Hanvey; M Shimizu; R D Wells
Journal:  Nucleic Acids Res       Date:  1990-01-11       Impact factor: 16.971

9.  Selective cleavage of human DNA: RecA-assisted restriction endonuclease (RARE) cleavage.

Authors:  L J Ferrin; R D Camerini-Otero
Journal:  Science       Date:  1991-12-06       Impact factor: 47.728

10.  Sequence-selective recognition of DNA by strand displacement with a thymine-substituted polyamide.

Authors:  P E Nielsen; M Egholm; R H Berg; O Buchardt
Journal:  Science       Date:  1991-12-06       Impact factor: 47.728
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  27 in total

Review 1.  Peptide nucleic acids: versatile tools for gene therapy strategies.

Authors:  D A Dean
Journal:  Adv Drug Deliv Rev       Date:  2000-11-15       Impact factor: 15.470

2.  Pseudocomplementary PNAs as selective modifiers of protein activity on duplex DNA: the case of type IIs restriction enzymes.

Authors:  Ekaterina Protozanova; Vadim V Demidov; Peter E Nielsen; Maxim D Frank-Kamenetskii
Journal:  Nucleic Acids Res       Date:  2003-07-15       Impact factor: 16.971

Review 3.  The peptide nucleic acids (PNAs): introduction to a new class of probes for chromosomal investigation.

Authors:  Franck Pellestor; Petra Paulasova
Journal:  Chromosoma       Date:  2004-05-19       Impact factor: 4.316

4.  Specific versus nonspecific binding of cationic PNAs to duplex DNA.

Authors:  Ayome Abibi; Ekaterina Protozanova; Vadim V Demidov; Maxim D Frank-Kamenetskii
Journal:  Biophys J       Date:  2004-05       Impact factor: 4.033

5.  Straightforward detection of SNPs in double-stranded DNA by using exonuclease III/nuclease S1/PNA system.

Authors:  Binzhi Ren; Jing-Min Zhou; Makoto Komiyama
Journal:  Nucleic Acids Res       Date:  2004-02-24       Impact factor: 16.971

6.  Site-selective and hydrolytic two-strand scission of double-stranded DNA using Ce(IV)/EDTA and pseudo-complementary PNA.

Authors:  Yoji Yamamoto; Akihiko Uehara; Takafumi Tomita; Makoto Komiyama
Journal:  Nucleic Acids Res       Date:  2004-11-01       Impact factor: 16.971

7.  SNP detection using peptide nucleic acid probes and conjugated polymers: applications in neurodegenerative disease identification.

Authors:  Brent S Gaylord; Michelle R Massie; Stuart C Feinstein; Guillermo C Bazan
Journal:  Proc Natl Acad Sci U S A       Date:  2004-12-23       Impact factor: 11.205

8.  Kinetic analysis of specificity of duplex DNA targeting by homopyrimidine peptide nucleic acids.

Authors:  V V Demidov; M V Yavnilovich; M D Frank-Kamenetskii
Journal:  Biophys J       Date:  1997-06       Impact factor: 4.033

Review 9.  Antisense peptide nucleic acids as a potential anti-infective agent.

Authors:  Hyung Tae Lee; Se Kye Kim; Jang Won Yoon
Journal:  J Microbiol       Date:  2019-05-27       Impact factor: 3.422

10.  Transcription-mediated binding of peptide nucleic acid (PNA) to double-stranded DNA: sequence-specific suicide transcription.

Authors:  H J Larsen; P E Nielsen
Journal:  Nucleic Acids Res       Date:  1996-02-01       Impact factor: 16.971
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