S Munné1, H U Weier, J Stein, J Grifo, J Cohen. 1. Gamete and Embryo Research Laboratory, New York Hospital-Cornell University Medical Center, New York 10021.
Abstract
PURPOSE: To use a 6-hr fluorescence in situ hybridization (FISH) procedure involving fluorochrome-labeled probes to determine the gender of blastomeres from arrested biopsied human embryos. RESULTS: Simultaneous detection of X and Y chromosomes was performed on 68 blastomeres with this technique. The FISH efficiency for gender determination was 95.5% (65/68). In addition, rehybridization with chromosome 8-specific probes was performed to determine the ploidy of blastomeres with more than two sex chromosomes. CONCLUSION: This technique offers an alternative to polymerase chain reaction for the preimplantation diagnosis of X-linked diseases and can also be used for ploidy assessment.
PURPOSE: To use a 6-hr fluorescence in situ hybridization (FISH) procedure involving fluorochrome-labeled probes to determine the gender of blastomeres from arrested biopsied human embryos. RESULTS: Simultaneous detection of X and Y chromosomes was performed on 68 blastomeres with this technique. The FISH efficiency for gender determination was 95.5% (65/68). In addition, rehybridization with chromosome 8-specific probes was performed to determine the ploidy of blastomeres with more than two sex chromosomes. CONCLUSION: This technique offers an alternative to polymerase chain reaction for the preimplantation diagnosis of X-linked diseases and can also be used for ploidy assessment.
Authors: M Plachot; A Veiga; J Montagut; J de Grouchy; G Calderon; S Lepretre; A M Junca; J Santalo; E Carles; J Mandelbaum Journal: Hum Reprod Date: 1988-07 Impact factor: 6.918
Authors: D K Griffin; A H Handyside; J C Harper; L J Wilton; G Atkinson; I Soussis; D Wells; E Kontogianni; J Tarin; S Geber Journal: J Assist Reprod Genet Date: 1994-03 Impact factor: 3.412