R J Zhang1, R A Wild, J M Ojago. 1. Department of Obstetrics and Gynecology, University of Oklahoma Health Sciences Center, Oklahoma City 73190.
Abstract
OBJECTIVE: To assess the effects of tumor necrosis factor-alpha (TNF-alpha) on adhesion of endometrial stromal cells to peritoneal mesothelial cells, a possible step leading to endometriosis. DESIGN: Analysis of cell adhesion in vitro. SETTING: University research laboratory. PATIENTS: Peritoneal fluid and biopsies were obtained from volunteers with regular cycles without endometriosis or endometrial pathology. INTERVENTIONS: Tumor necrosis factor-alpha (0.1 to 1,000 U/mL) was added to nutrient media with the following supplements: 10% fetal calf serum, epidermal growth factor 20 ng/mL, and gentamicin 10 ng/mL. MAIN OUTCOME MEASURES: Radioactivity of chromium-51 was used as a reflection of adherent stromal cells. RESULTS: The adherence of endometrial stromal cells to mesothelial cells was significantly increased by pretreatment of mesothelial cells with TNF-alpha. CONCLUSIONS: Tumor necrosis factor-alpha may play a facilitory role in the development of endometriosis.
OBJECTIVE: To assess the effects of tumor necrosis factor-alpha (TNF-alpha) on adhesion of endometrial stromal cells to peritoneal mesothelial cells, a possible step leading to endometriosis. DESIGN: Analysis of cell adhesion in vitro. SETTING: University research laboratory. PATIENTS: Peritoneal fluid and biopsies were obtained from volunteers with regular cycles without endometriosis or endometrial pathology. INTERVENTIONS:Tumor necrosis factor-alpha (0.1 to 1,000 U/mL) was added to nutrient media with the following supplements: 10% fetal calf serum, epidermal growth factor 20 ng/mL, and gentamicin 10 ng/mL. MAIN OUTCOME MEASURES: Radioactivity of chromium-51 was used as a reflection of adherent stromal cells. RESULTS: The adherence of endometrial stromal cells to mesothelial cells was significantly increased by pretreatment of mesothelial cells with TNF-alpha. CONCLUSIONS:Tumor necrosis factor-alpha may play a facilitory role in the development of endometriosis.
Authors: Fan Mu; Holly R Harris; Janet W Rich-Edwards; Susan E Hankinson; Eric B Rimm; Donna Spiegelman; Stacey A Missmer Journal: Am J Epidemiol Date: 2018-03-01 Impact factor: 4.897
Authors: Wei Hao; Ivy H Y Chan; Xuelai Liu; Paula M Y Tang; Paul K H Tam; Kenneth K Y Wong Journal: Pediatr Surg Int Date: 2011-10-13 Impact factor: 1.827