Vaccination of bovines against Schistosomiasis japonica with cryopreserved-irradiated and freeze-thaw schistosomula.

Four laboratory tests and one field trial with cryopreserved irradiated (CI) schistosomula vaccine and a freeze-thaw (F/T) vaccine against bovine Schistosomiasis japonica were carried out in 1979 and 1980 with the following results: (1) Single intradermal vaccination in buffalo calves each with 10,000 20 krad CI Schistosomula plus 1 ml BCG gave 62% worm reduction (P < 0.05). Using the same protocol 55% worm reduction (P < 0.01) was obtained in cattle. (2) Buffalo calves immunized twice, at a 1.5 month interval, with 10,000 and 20,000 CI schistosomula, respectively, resulted in a worm reduction 65%. (3) In a preliminary field trial with 10,000 CI schistosomula plus 1 ml BCG resulted in a worm reduction of 53% in buffalo calves. (4) Intradermal vaccination of 30,000 F/T schistosomula with 1 ml BCG was also tried in cattle and revealed a worm reduction of 57% but increasing the number of vaccinations did not improve the protective effect. (5) Evidence regarding the effects of immunization with CI vaccine in buffaloes and F/T vaccine in cattle, on the number of eggs and miracidia and that of female worms themselves was obtained. (6) Immune responses, cellular and humoral, elicited in buffaloes vaccinated with CI schistosomula were detected by means of Lymphocyte Transformation Assay and Enzyme Linked Immunosorbent Assay.