Literature DB >> 849277

The turnover of hamster fibroblast lysosomal beta-D-glucuronidase.

M J Warburton, C H Wynn.   

Abstract

The half-life of hamster fibroblast beta-D-glucuronidase (EC 3.2.1.31) was estimated to be 4-5 days by measuring the decay with time of the radioactivity in beta-D-glucuronidase isolated from cells grown in the presence of 14C-labelled amino acids. A new affinity-chromatographic procedure for the purification of beta-D-glucuronidase is described.

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Year:  1977        PMID: 849277      PMCID: PMC1164585          DOI: 10.1042/bj1620201

Source DB:  PubMed          Journal:  Biochem J        ISSN: 0264-6021            Impact factor:   3.857


  13 in total

1.  Concerning a possible mechanism for selective capture of cytoplasmic proteins by lysosomes.

Authors:  R T Dean
Journal:  Biochem Biophys Res Commun       Date:  1975-11-17       Impact factor: 3.575

2.  The preparation and properties of beta-glucuronidase. IV. Inhibition by sugar acids and their lactones.

Authors:  G A LEVVY
Journal:  Biochem J       Date:  1952-11       Impact factor: 3.857

3.  The hyperactivity of hamster fibroblast lysosomal enzymes after endocytosis of sucrose.

Authors:  M J Warburton; C H Wynn
Journal:  Biochem Biophys Res Commun       Date:  1976-05-03       Impact factor: 3.575

4.  Characterisation of the lysosomal heterogeneity in Chinese-hamster fibroblasts.

Authors:  M J Warburton; C H Wynn
Journal:  Eur J Biochem       Date:  1976-06-01

Review 5.  Intracellular protein degradation in mammalian and bacterial cells.

Authors:  A L Goldberg; J F Dice
Journal:  Annu Rev Biochem       Date:  1974       Impact factor: 23.643

6.  Relationship between digradation rates of proteins in vivo and their susceptibility to lysosomal proteases.

Authors:  H I Segal; J R Winkler; M P Miyagi
Journal:  J Biol Chem       Date:  1974-10-10       Impact factor: 5.157

7.  Purification of beta-glucosidase activities from bovine spleen by affinity chromatography.

Authors:  J N Kanfer; R A Mumford; S S Raghavan; J Byrd
Journal:  Anal Biochem       Date:  1974-07       Impact factor: 3.365

8.  Synthesis and turnover of lysosomal glycoproteins. Relation to the molecular heterogeneity of the lysosomal enzymes.

Authors:  A Goldstone; H Koenig
Journal:  FEBS Lett       Date:  1974-02-15       Impact factor: 4.124

9.  Purification of alpha- and beta-galactosidases by affinity chromatography.

Authors:  J N Kanfer; G Petrovich; R A Mumford
Journal:  Anal Biochem       Date:  1973-09       Impact factor: 3.365

10.  The effect of intralysosomal sucrose storage on the turnover of hamster fibroblast lysosomal and Golgi-apparatus enzymes.

Authors:  M J Warburton; C H Wynn
Journal:  Biochem J       Date:  1976-08-15       Impact factor: 3.857

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  7 in total

1.  Acid hydrolase activity in red and white skeletal muscle of mice during a two-week period following exhausting exercise.

Authors:  V Vihko; A Salminen; J Rantamäki
Journal:  Pflugers Arch       Date:  1978-12-28       Impact factor: 3.657

2.  Purification and immunological quantification of rat liver lysosomal glycosidases.

Authors:  P C de Groen; G D LeSage; P S Tietz; N F LaRusso
Journal:  Biochem J       Date:  1989-11-15       Impact factor: 3.857

3.  Evidence for lysosomal enzyme recognition by human fibroblasts via a phosphorylated carbohydrate moiety.

Authors:  K Ullrich; G Mersmann; E Weber; K Von Figura
Journal:  Biochem J       Date:  1978-03-15       Impact factor: 3.857

4.  Heterogeneity of lysosomal enzymes in cultured normal and sialidosis type II human fibroblasts and the effect of ammonium chloride on this heterogeneity.

Authors:  C M Heyworth; C H Wynn
Journal:  Mol Cell Biochem       Date:  1985-05       Impact factor: 3.396

5.  Turnover of kidney beta-glucuronidase in normal and Chédiak-Higashi (beige) mice.

Authors:  R T Swank; E J Brandt
Journal:  Am J Pathol       Date:  1978-09       Impact factor: 4.307

6.  Turnover of beta-galactosidase in fibroblasts from patients with genetically different types of beta-galactosidase deficiency.

Authors:  O P Van Diggelen; A W Schram; M L Sinnott; P J Smith; D Robinson; H Galjaard
Journal:  Biochem J       Date:  1981-10-15       Impact factor: 3.857

7.  Specific inactivation of lysosomal glycosidases in living fibroblasts by the corresponding glycosylmethyl-p-nitrophenyltriazenes.

Authors:  O P Van Diggelen; H Galjaard; M L Sinnott; P J Smith
Journal:  Biochem J       Date:  1980-05-15       Impact factor: 3.857

  7 in total

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